ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe (1981)

Inoue, Hirokazu ; Shimoda, Chikashi

Springer

Archives of microbiology 129 (1981), S. 19-22

add to mindlist on the mindlist

Details

ISSN: 1432-072X

Keywords: Germination ; Glycogen ; Outgrowth ; Schizosaccharomyces pombe ; Spore ; Trehatase ; Trehalose ; Yeast

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC 3.2.1.28) was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00417172

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV) (1990)

Oka, Takashi ; Yutsudo, Masuo ; Inoue, Hirokazu ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 145 (1990), S. 428-433

add to mindlist on the mindlist

Details

ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. Molecular Biological analysis of thre revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. These findings suggest the existence of a specific mechanism(s) for elimination of the v-abl oncogene by segregation, mutation, or gene rearrangement in these cells.

Additional Material: 5 Ill.