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  • 1
    Electronic Resource
    Electronic Resource
    Intervarietal and interspecific chimera formation by in vitro graft-culture method in Brassica (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 727-732 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Chimera ; Tissue culture ; Graft
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An efficient chimera formation method by tissue culture combined with grafting was studied in Brassica. Cabbage cultivars “Ruby-ball” and “YR-ranpou” (Brassica oleracea) were used for intervarietal chimera formation. Seven-day-old seedlings leaving one of two cotyledons were approach-grafted and cultured in vitro (AGSC method). Chimeric shoots were obtained by the subculture of directly growing chimeric leaves (DGCL) from grafted part and cross-cut section of the united part after the graft culture. These were rooted and grown to complete chimera. An approach-grafted culture method was also available for interspecific chimera formation between “Komatsuna” (B. campestris) and “Ruby-ball” (B. oleracea), resulting in 20% formation per culture. Interspecific chimeras were identified as complex and peripheral-sectorial type by microscopic observation and the electrophoretic analysis of acid phosphatase isozyme pattern. The AGSC method was more effective than the usual Winkler's graft method and a simple mixed culture of heterogeneous cells or tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226691
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Graft-induced change in fruit shape in Capsicum annuum L. I. genetic analysis by crossing (1987)
    Springer
    Euphytica 36 (1987), S. 809-814 
    Details
    ISSN: 1573-5060
    Keywords: Capsicum annuum ; pepper ; graft hybrid ; fruit shape ; nuclear gene change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Characteristics of fruit shape in the progeny from a graft-induced change were analysed. Non-pointed, typically inverted-blunt (two-lobed) fruit apex was observed in chimeric state within a scion of Yatsubusa grafted onto Spanish Paprika (inverted-blunt, three-or four-lobed fruit). This frequency of change in fruit shape was unstable in an early generation on the graft.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051864
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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