ISSN:
1432-0428
Keywords:
Keywords Insulin-dependent diabetes mellitus
;
HLA class II alleles
;
newborn screening
;
autoimmunity.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Autoimmunity causing insulin-dependent diabetes mellitus (IDDM) begins in early childhood due to interactions between genes and unknown environmental factors that may be identified through follow-up of a large cohort of genetically susceptible children. Such a cohort has been established using a simple and rapid cord blood screening for HLA alleles. The DRB1 and DQB1 second exon sequences were co-amplified using the polymerase chain reaction and hybridized with single and pooled sequence-specific oligonucleotide probes. Four individual probes were used to detect the susceptibility alleles DRB1*03, DRB1*04, and DQB1*0302 as well as the usually protective DRB1*15/16 (DR2) alleles. In addition, pooled probes allow the distinction of DR3/3 from the DR3/x genotype (where x is neither DR2, 3, nor 4) and DR4/4 from DR4/x. Among 5000 newborns from the general Denver population, we have found the high-risk genotype (DRB1*03/DRB1*04, DQB1*0302) to be present in 2.4 % of non-Hispanic whites, 2.8 % of Hispanics, and 1.6 % of African Americans. The moderate-risk genotypes (DRB1*04, DQB1*0302/DRB1*04, DQB1*0302, DRB1*04, DQB1*0302/x, or DRB1*03/DRB1*03) are present in 17 % of American non-Hispanic whites, 24 % of Hispanics and in 10 % of African Americans. These results demonstrate the feasibility of a large-scale newborn screening for genes associated with IDDM. The ultimate role for such a screening in future routine prediction and prevention of IDDM will depend on the availability of an effective and acceptable form of clinical intervention. [Diabetologia 1996) 39: 807–812]
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001250050514
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