ISSN:
1573-7217
Keywords:
breast cancer
;
dot (slot) blot hybridization
;
HPV DNA
;
mammary Paget's disease
;
Paget's disease
;
PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The paraffin sections from 20 nipples with Paget's disease (10 central intraductal and 10 invasive ductal carcinomas) were analyzed for human papilloma virus (HPV) DNA of the low- and intermediate/high-risk groups. Polymerase chain reaction (PCR) and dot (slot) blot hybridization were used for the detection of HPV DNA types 6/11/16/18/31/33/35. In addition, we examined the c-erbB-2 oncogene expression in the specimens to differentiate benign cells in the surface epithelium of the nipple and areola from Paget cells. We found that the oncogene expression of the c-erbB-2 displayed a strong signal in the Paget cells. Using PCR and dot (slot) blot hybridization, we could not detect the HPV DNAs that are specific for the low- and intermediate/high riskgroups in the 20 cases of Paget's disease. Our results showed for the first time that this type of virus did not contribute to the pathogenesis of Paget's disease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01807036
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