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  • Life Sciences  (12)
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  • 11
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lactic acid and short-chain carboxylic acids C1-C5 were analyzed on an Aminex HPX-87H column using UV and RI detection. The HPLC method was applied to complex fermentation media of a microbial lactic acid formation process. The resulting chromatograms of the yeast extract or culture medium showed some UV-absorbing substances that interfered partially with peaks of short-chain carboxylic acids.In contrast to UV monitoring, RI detection gave only a few small peaks of these so-called “background chromatograms”. Also glucose used as substrate could be quantified in the culture medium, because of which RI detection should be preferred.Furthermore, a self-prepared cation-exchange resin (SAC = S-DVB) based on a poly(styrenedivinylbenzene) partially comparable in its properties with Aminex resins was useful for fast (about 4 minutes) and not too expensive determinations of lactic and acetic acid in fermentation media.Applying this separattion system to fermentation solutions detected by RI as well as UV monitoring results in the quantitative analysis of lactic acid kinetics identical with an Aminex HPX-87H column were achieved.Finally, the simplicity of lactic-acid analysis is illustrated by examples of dairy products only centrifugated and diluted in double distilled water before injecting on a cation-exchange column.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 7 (1987), S. 237-245 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In commercial batch processes the productivity of product formation is low. But a significant increase of productivity can be achieved in continuous fermentations. By using high flow rate fermenter systems characterized by a relatively long retention time of biomass in comparison with the retention time of the liquid we can realize a high-performance fermentation. The problem of holding back the biomass within the reactor could be solved by means of membranes being impenetrable to the cells, but permeable to the hydraulic phase. Such a process technology was successfully tested for its applicability in alcoholic and lactic acid fermentations. The maximum productivities obtained on this way were ṗ = 120 g/l. · h for ethanol production and ṗ = 51 g/l. h for lactic acid fermentation, respectively.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ethanol-inhibitory behaviour of the yeast Saccharomyces cerevisiae Sc 5 was found to be characterized by a continual-linear relation between the specific ethanol formation rate and the ethanol concentration. Therefore the simple equation \documentclass{article}\pagestyle{empty}\begin{document}$$v = v_0 - a \cdot P$$\end{document} could be applied for it.It is shown that this model is correct only then, if all of the process parameters are in their optimum.Out of the optimum temperature range the characteristics of the function ν = f(P) change in such a way that in regard to the ethanol concentration P twc linear relations exist for each suboptimum temperature: \documentclass{article}\pagestyle{empty}\begin{document}$$ \begin{array}{*{20}c} {v_T = v'_{0T} - a_0 \cdot e^{nT} \cdot P,} & {{\rm for}} & {P 〈 P_T } & {{\rm and}} \\\end{array} $$\end{document} \documentclass{article}\pagestyle{empty}\begin{document}$$ \begin{array}{*{20}c} {v_T = v_0 - a \cdot P,} & {{\rm for}} & {P 〉 P_T ,} \\\end{array} $$\end{document} and a non-linear equation is current for each superoptimum temperature: \documentclass{article}\pagestyle{empty}\begin{document}$$ v_T = v'_{0T} - a_0 ^\prime \cdot e^{mT} \cdot P^{b_T } , $$\end{document} where bT is also a function of the temperature and always less than 1.Taking as a basis these equations the specific ethanol formation rate of the used strain can be calculated for the whole biokinetic P/T-sphere of ethanol production.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 14
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 431-439 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The shape of the temperature profile curve of ethanol production changes in the presence of ethanol. So the characteristic points of the curve are shifted to lower values at increasing ethanol concentrations. As a reason for it the potentiation of thermal deactivation effects by ethanol already at lower temperatures is assumed. Compared to it, the activation energy of ethanol for mation in the suboptimal temperature range was found to be unaffected by alcohol.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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