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  • High resolution map  (2)
  • Key words Adaptability  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Genetic analysis of the dwarfing gene Rht8 in wheat. Part II. The distribution and adaptive significance of allelic variants at the Rht8 locus of wheat as revealed by microsatellite screening (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1110-1120 
    Details
    ISSN: 1432-2242
    Keywords: Key words Adaptability ; Dwarfing gene (Rht8) ; Microsatellite ; Molecular markers ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Wheat microsatellite WMS 261 whose 192-bp allele has been shown to be diagnostic for the commercially important dwarfing gene Rht8 was used to screen over 100 wheat varieties to determine the worldwide spread of Rht8. The results showed Rht8 to be widespread in southern European wheats and to be present in many central European wheats including the Russian varieties ‘Avrora’, ‘Bezostaya’ and ‘Kavkaz’. Rht8 appears to be of importance to South European wheats as alternative giberellic acid (GA)-insensitive dwarfing genes do not appear to be adapted to this environment. The very successful semi-dwarf varieties bred by CIMMYT, Mexico, for distribution worldwide have been thought to carry Rht8 combined with GA-insensitive dwarfing genes. Additional height reduction would have been obtained from pleiotropic effects of the photoperiod-response gene Ppd1 that is essential to the adaptability of varieties bred for growing under short-winter days in tropical and sub-tropical areas. The microsatellite analysis showed that CIMMYT wheats lack Rht8 and carry a WMS 261 allelic variant of 165 bp that has been associated with promoting height. This presumably has adaptive significance in partly counteracting the effects of other dwarfing genes and preventing the plants being too short. Most UK, German and French wheats carry an allelic variant at the WMS 261 locus with 174 bp. This could be selected because of linkage with the recessive photoperiod-sensitive ppd1 allele that is thought to offer adaptive significance northern European wheats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050846
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of a high-resolution genetic map and YAC-contigs in the tomato Tm-2a region (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 228-233 
    Details
    ISSN: 1432-2242
    Keywords: Key words Tomato ; TMV resistance ; RFLP ; High resolution map ; YAC contig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract With the ultimate goal of cloning the Tobacco Mosaic Virus (TMV) resistance gene Tm-2a from tomato by means of positional cloning, a high-resolution map of a 4.3-cM region surrounding the Tm-2a gene has been constructed. In total, 13 RFLP and RAPD markers were mapped in close proximity to Tm-2a using 2112 individuals from an intraspecific Lycopersicon peruvianum backcross. The closest flanking markers were separated from Tm-2a by 0.05 cM on each side. Only one marker, the cDNA clone R12, co-segregated with Tm-2a. In order to physically cover the Tm-2a region, R12 and the flanking DNA marker TG207 were used to select homologous YAC clones. To-date, two YAC-contigs spanning approximately 340 kb and 360 kb have been constructed. The data obtained from these experiments indicate that recombination around the centromere of chromosome 9 is extremely suppressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050270
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Construction of a high-resolution genetic map and YAC-contigs in the tomato Tm-2a region (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 228-233 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; TMV resistance ; RFLP ; High resolution map ; YAC contig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract With the ultimate goal of cloning the Tobacco Mosaic Virus (TMV) resistance gene Tm-2a from tomato by means of positional cloning, a high-resolution map of a 4.3-cM region surrounding the Tm-2a gene has been constructed. In total, 13 RFLP and RAPD markers were mapped in close proximity to Tm-2a using 2112 individuals from an intraspecific Lycopersicon peruvianum backcross. The closest flanking markers were separated from Tm-2a by 0.05 cM on each side. Only one marker, the cDNA clone R12, co-segregated with Tm-2a. In order to physically cover the Tm-2a region, R12 and the flanking DNA marker TG207 were used to select homologous YAC clones. To-date, two YAC-contigs spanning approximately 340 kb and 360 kb have been constructed. The data obtained from these experiments indicate that recombination around the centromere of chromosome 9 is extremely suppressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225750
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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