ISSN:
1432-0797
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Experiments using a soluble, chemically defined fermentation medium provided important knowledge about the kinetics of efrotomycin biosynthesis. Equivalent titers were obtained in a batch process in both shaker flasks and fermentors. By extended feeding of both monosodium glutamate and glycerol at elevated temperatures, in combination with sulphuric acid pH control, the specific production rate was increased 2.8 fold and overall production rate was improved 5-fold. If the monosodium glutamate was fed too fast, then ammonium accumulated with indications of strong repression of efrotomycin biosynthesis at concentrations above 6 mM. In contrast to the complex medium used for this process, the chemically define medium was completely insensitive to changes in sterilization conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00386235
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