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  • 1
    Electronic Resource
    Electronic Resource
    Acute toxicity of pyridostigmine in rats: histological findings (1986)
    Springer
    Archives of toxicology 58 (1986), S. 271-275 
    Details
    ISSN: 1432-0738
    Keywords: Pyridostigmine ; Acute toxicity ; Histopathology of skeletal muscles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To assess the morphological changes due to pyridostigmine (Pyr), sublethal doses of Pyr (20 and 40 mg/ kg body weight) were given to adult rats through a gastric tube. Within 24 h, acute focal necroses, leukocytic infiltrates and marked changes in the motor endplates appeared in the skeletal muscle. The changes were more evident in the diaphragm than in the quadriceps muscle. The acetylcholinesterase activity in the whole blood and in the erythrocytes was reduced to considerably less than onehalf of the normal value. The histological changes induced by Pyr have not been reported to date, but are similar to those observed in experimental poisoning with other anticholinesterase agents such as carbamates and organophosphates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297119
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunolocalization of Na/SO4-cotransport (NaSi-1) in rat kidney (1996)
    Springer
    Pflügers Archiv 432 (1996), S. 373-378 
    Details
    ISSN: 1432-2013
    Keywords: Key words Kidney ; Proximal tubules ; Sodium-dependent sulphate cotransport ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The proximal tubule is the major site for renal reabsorption of sulphate. A sodium-dependent transport system for sulphate (NaSi-1) has recently been identified from a rat kidney cortex cDNA library. Recent work demonstrated that NaSi-1 mRNA is expressed predominantly in proximal tubules. In the present work expression along the nephron of the Na/SO4-cotransporter NaSi-1 was studied by immunofluorescence. A polyclonal antibody was raised in rabbits against a fusion protein containing a 53-amino-acid polypeptide specific for the NaSi-1 sequence. The anti-NaSi-1 polyclonal antibody specifically detected a 68-kDa protein on Western blots and, by immunofluorescence specific staining, was observed in MDCK cells transfected with the NaSi-1 cotransporter. Using rat kidney cortex slices specific NaSi-1-related immunoreactivity was detected in proximal tubules and was restricted to the apical membrane. No immunoreactivity was observed in the other nephron segments. This was confirmed by Western blot analysis using proximal tubular apical and basolateral membranes isolated by free-flow electrophoresis. The results indicate that the Na/SO4-cotransporter NaSi-1 is expressed in the apical membrane of proximal tubular cells and is therefore likely to be involved in proximal reabsorption of sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050147
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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