Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Schizophyllum  (4)
  • Homeodomain  (3)
  • Key words Schizophyllum commune  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Organization of the ribosomal RNA genes of Schizophyllum commune (1988)
    Springer
    Current genetics 13 (1988), S. 417-424 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum ; Ribosomal DNA ; Gene organization ; Length polymorphisms ; Transcriptional initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 18, 5.8, 25 and 5S ribosomal RNA (rRNA) cistrons have been mapped on the ribosomal DNA (rDNA) unit repeat of Schizophyllum commune strain 4–40. These genes are spatially ordered in the sequence given. The presence of a large primary precursor rRNA which is processed to form the mature 18, 5.8 and 25S rRNAs has been demonstrated. We have mapped the site of transcriptional initiation for this rRNA primary precursor. The sequence surrounding this site has been determined and shown to be highly conserved, with considerable identity to those in Neurospora crassa and Dictyostelium discoideum. The direction of transcription of the rRNA genes has been determined. The 5S rRNA cistron is transcribed in the same direction as the other rRNAs, however it is not transcribed as a part of the large primary precursor. The previously identified rDNA strain-specific length polymorphisms (Specht et al. 1984) are shown to be located within the transcribed region of the rDNA unit repeat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365663
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Developmental regulation of the methylation of the ribosomal DNA in the basidiomycete fungusSchizophyllum commune (1988)
    Springer
    Current genetics 14 (1988), S. 105-111 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum ; Methylation ; Ribosomal DNA ; Developmental regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ribosomal DNA (rDNA) ofSchizophyllum commune was shown previously to be highly methylated (Specht et al. 1984). To investigate the role of methylation of rDNA, a map of the recognition sites (CCGG) of the methylation-sensitive restriction endonucleases, MspI and Hpall, was prepared for the rDNA ofS. commune strain 4–40. The incidence of methylation at these CCGG sequences was analyzed. Our analyses demonstrate that either of the cytosines of the CCGG sequence can be methylated in Schizophyllum, however methylation of the internal cytosine is more frequent than methylation of the external cytosine. The external cytosines of CCGG sequences near the rDNA transcriptional initiation site are less frequently methylated than those else-where in the rDNA. The methylation of these sequences has been shown to be developmentally regulated inS. commune. We find that these sequences are more highly methylated in dikaryonic than homokaryotic cells. We propose that methylation of the rDNA may have a role in regulating the transcription of rRNA inS. commune.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569333
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cloning and characterization of aSchizophyllum gene withAβ6 mating-type activity (1996)
    Springer
    Current genetics 29 (1996), S. 136-142 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Mating type ; Homeodomain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The A-pathway of development in the basidiomycete fungusSchizophyllum commune may be activated by either of two mating-type loci,Aξ andAβ Aα consists of two multiallelic genes,Y andZ. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non- self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci,Aα andAβ, both manage to regulate the same pathway. Here we report the structure of a gene withAβ6 activity. This gene, denotedAβV6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in Aα Y1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to AαY, AαZ, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus whileAα andAβ encode different proteins, the general mechanism by which Aα and Aβ components signal A-regulated development may be similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221577
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Cloning and characterization of a Schizophyllum gene with Aβ6 mating-type activity (1996)
    Springer
    Current genetics 29 (1996), S. 136-142 
    Details
    ISSN: 1432-0983
    Keywords: Key words Schizophyllum commune ; Mating type ; Homeodomain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, Aα and Aβ. Aα consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci, Aα and Aβ, both manage to regulate the same pathway. Here we report the structure of a gene with Aβ6 activity. This gene, denoted AβV6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in Aα Y1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to AαY, AαZ, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus while Aα and Aβ encode different proteins, the general mechanism by which Aα and Aβ components signal A-regulated development may be similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050028
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA of Schizophyllum commune: restriction map, genetic map, and mode of inheritance (1992)
    Springer
    Current genetics 22 (1992), S. 129-134 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA ; Fungi ; Basidiomycete ; Schizophyllum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA (mtDNA) found in the basidiomycete Schizophyllum commune (strain 4–40) is a circular molecule 49.75 kbp in lenght. A physical map containing 61 restriction sites revealed no repeat structures. Cloned genes from Neurospora crassa, Aspergillus nidulans, and Saccharomyces cerevisiae were used in Southern hybridizations to locate nine mitochondrial genes, including a possible pseudogene of ATPase 9, on the restriction map. A probe from a functional ATPase 9 gene identified homologous fragments only in the nuclear genome of S. commune. Restriction fragment length polymorphisms (RFLPs) between mtDNA isolated from different strains of S. commune were used to show that mitochondria do not migrate with nuclei during dikaryosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351472
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Only one of the paired Schizophyllum commune Aα mating-type, putative homeobox genes encodes a homeodomain essential for Aα-regulated development (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 318-324 
    Details
    ISSN: 1617-4623
    Keywords: Schizophyllum commune ; Homeodomain ; Homeobox ; Mating type
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aα mating-type locus is one of four master regulatory loci controlling sexual development in Schizophyllum commune. The Aα locus contains two homeobox genes, Y and Z, encoding two homeodomain-related proteins, Y and Z, Y and Z are each multi-allelic genes. When haploid strains form fusion cells, only particular combinations of Y and Z alleles activate Aα-regulated sexual development. The role of the putative homeodomain was examined in several Y and Z alleles by site-directed mutagenesis of regions critical to secondary structure and function of homeodomains. Mutations of the Z homeobox do not affect the function of Z proteins in Aα-activated development, but mutations of Yhomeoboxes destroy the ability of Y proteins to activate development. We conclude that only one of two Aα homeodomain-related regulators relies upon the homeodomain motif to effect gene expression in sexual development. This conclusion affords a refinement of our working hypothesis for the mechanism by which Aα proteins may regulate target gene expression. On the basis of our results with the Z protein, we speculate that the DNA-binding motifs of some transcriptional regulators may be lost or modified during evolution once these regulators have been recruited to participate in complexes with other DNA-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285460
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Transformation of the basidiomycete,Schizophyllum commune (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 103-106 
    Details
    ISSN: 1617-4623
    Keywords: Schizophyllum ; Basidiomycete ; Transformation ; tryptophan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts of aSchizophyllum commune tryptophan auxotroph (trp1), deficient in indole-3-glycerol phosphate synthetase (IGPS), were transformed to trp+ with plasmid DNA containing the SchizophyllumTRP1 sequence. Efficiencies up to 30 transformants per microgram of plasmid DNA were obtained. Southern blots reveal that the transforming DNA is integrated in chromosomal DNA. The trp+ phenotype of transformants is stable in meiosis and mitosis. Transformants possess IGPS activity comparable to wild-type cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02428038
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...