ISSN:
1433-8580
Keywords:
IgM
;
Purification
;
Methods
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary For the preparation of gram amounts of IgM from human sera sedimention at 100,000g or treatment with ZnSO4 of the redissolved “euglobulin”-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01851630
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