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  • 1
    Electronic Resource
    Electronic Resource
    Neurovirulence and latency in inbred mice of two HSV-1 intrastrain variants of divergent pathogenicity (1986)
    Springer
    Medical microbiology and immunology 174 (1986), S. 313-324 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The pathogenicity pattern of the HSV-1 strain ANG which is nonencephalitogenic in mice is compared with that of a selected neurovirulent variant of this strain in DBA-2 mice. After i.p. inoculation both variants replicate to high titers in the mouse peritoneum and build up a virus reservoir in the spleen. Both viruses have no effect on visceral mouse organs other than the spleen; both viruses lead to an inefficient and masked viraemia and both replicate efficiently in CNS tissue after direct intracranial injection. Only the pathogenic variant, however, spreads to the CNS and leads to lethal encephalitis upon intraperitoneal infection. The assumption that infection of the CNS would be mediated by hematogenous transport is not supported by the data obtained from transfer and cocultivation experiments with lymphocytes or experiments involving artificial viraemia. In a model to analyse the capacity of the viruses to invade nerve axons and to induce a latent infection both viruses were found to be latency positive in dorsal root ganglia. It is clear that non-neurovirulent HSV-1 strains are subjected to a postganglionic block of virus spread from the periphery to the CNS. The experiments led to the hypothesis that axonal transport even beyond the dorsal root ganglia to the CNS proceeds unrestricted, whereas lethal CNS invasion is prevented by a restriction of viral replication of HSV-1 ANG in the CNS by a virus-induced host defence mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02123684
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Large-scale purification of IgM from human sera (1980)
    Springer
    Research in experimental medicine 177 (1980), S. 33-41 
    Details
    ISSN: 1433-8580
    Keywords: IgM ; Purification ; Methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary For the preparation of gram amounts of IgM from human sera sedimention at 100,000g or treatment with ZnSO4 of the redissolved “euglobulin”-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851630
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A simple and efficient method for preparation of immunoelectrophoretically pure guinea pig IgM and isolation of monospecific anti-IgM antibodies (1979)
    Springer
    Research in experimental medicine 175 (1979), S. 279-286 
    Details
    ISSN: 1433-8580
    Keywords: Anti-μ-chain antibodies ; Immunoadsorption on beaded agarose ; Guinea pig IgM purification ; Separation of guinea pig immunoglobulin classes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This report describes experimental conditions developed for the rapid and efficient preparation of guinea pig IgM in a purified form and in high yield. A combined immunization procedure had to be designed to obtain sufficiently high IgM concentrations in guinea pig serum. The isolation procedure includes precipitation at low pH, followed by sedimentation at 110,000 g and reverse flow gel chromatography on Sephadex G-200. Furthermore, we describe in detail the isolation of relatively large amounts of pure and specific anti-guinea pig μ-chain antibodies by immunoaffinity chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851284
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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