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  • Immunohistochemistry  (2)
  • Spinal cord  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Further analysis of presence of peptides in dopamine neurons (1988)
    Springer
    Experimental brain research 72 (1988), S. 523-534 
    Details
    ISSN: 1432-1106
    Keywords: Neuropeptides ; Tyrosine hydroxylase ; Colocalization ; Coexistence ; Hypothalamus ; Periaqueductal grey matter ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Double-labeling combined with elution-restaining immunofluorescence techniques were used to analyze the extent of coexistence among the peptides cholecystokinin (CCK), peptide histidine-isoleucine (PHI)/vasoactive intestinal polypeptide (VIP), substance P and the catecholamine-synthesizing enzyme tyrosine hydroxylase in neurons of the supramammillary region and mesencephalon of the rat. Approximately 50% of the PHI/VIP-containing perikarya and about 25% of the CCK-positive cell bodies in the supramammillary region exhibited coexistence of both peptides. Only a very minor portion of these double-labeled neurons were also found to contain immunostaining for tyrosine hydroxylase (indicative of dopamine in these cells). A low percentage of the neurons contained the enzyme plus either CCK- or PHI/VIP-like immunoreactivity. A low proportion of the tyrosine hydroxylase-positive neurons in this region contained substance P-like immunoreactivity and vice versa. In other areas, small numbers of neurons in periventricular and periaqueductal regions were found to be immuno-stained for CCK, PHI/VIP and tyrosine hydroxylase. Single examples of triple-labeled (CCK-PHI/VIP-TH) somata were infrequently observed in the ventral tegmental area. These data provide further evidence of peptide/peptide and peptide/monoamine coexistence in the central nervous system. The demonstration of CCK-PHI/VIP colocalization (possibly including a minor dopaminergic component) and of substance P and tyrosine hydroxylase coexistence within neurons of the supramammillary region, which has widespread projections to many areas of the forebrain, suggests that these neuropeptides may coexist in some of these pathways and perhaps be co-released in several different regions of the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00250598
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Thyrotropin releasing hormone (TRH)-immunoreactive cell groups in the rat central nervous system (1987)
    Springer
    Experimental brain research 68 (1987), S. 213-217 
    Details
    ISSN: 1432-1106
    Keywords: Neuropeptide ; Brain ; Spinal cord ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using a paraformaldehyde-picric acidglutaraldehyde-containing fixative and treatment of the tissue with sodium borohydride, numerous and widespread TRH-immunoreactive cell bodies were observed in the central nervous system of colchicinetreated rats, including the olfactory bulb, cortical and hippocampal areas, the caudate nucleus and other subcortical areas, many hypothalamic nuclei, the periaqueductal central gray, pontine nuclei, medulla oblongata and the dorsal horn of the spinal cord. Most of these cells could not be visualized with the same antiserum when conventional fixation methods based on formalin alone were used. The present findings suggest that TRH systems are considerably more extensive than hitherto assumed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00255248
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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