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Raman spectroscopic studies on the interaction between divalent counterion and polyion

Koda, S. ; Nomura, H. ; Nagasawa, M.

Amsterdam : Elsevier

Biophysical Chemistry 18 (1983), S. 361-367

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ISSN: 0301-4622

Keywords: Poly(acrylic acid): Ion binding ; Roman spectroscopy

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology , Chemistry and Pharmacology , Physics

Type of Medium: Electronic Resource

URL: http://linkinghub.elsevier.com/retrieve/pii/0301-4622(83)80049-0

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Clinical significance of U1-RNP immune complexes in mixed connective tissue disease and systemic lupus erythematosus (1987)

Negoro, N. ; Kanayama, Y. ; Takeda, T. ; [et al.]

Springer

Rheumatology international 7 (1987), S. 7-11

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ISSN: 1437-160X

Keywords: U1-ribonucleoprotein ; Immune complexes ; Mixed connective tissue disease ; Systemic lupus erythematosus

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary We measured U1-RNP: anti-U1-RNP immune complexes (U1-RNP ICs) in patients with mixed connective tissue disease (MCTD) and systemic lupus erythematosus (SLE) to examine the clinical significance of circulating U1-RNP ICs. The level of U1-RNP ICs in 11 patients with MCTD was significantly higher than that in 22 normal subjects and there was a close correlation between the level of U1-RNP ICs and the clinical disease activity index of MCTD. In contrast, the level of U1-RNP ICs in 31 patients with SLE was not significantly higher than that in normal subjects and that was not correlated with the clinical disease activity index of SLE or the renal histologic activity index of lupus nephritis. We conclude that U1-RNP ICs are present in sera of patients with MCTD and SLE, and that the level of U1-RNP ICs may be closely associated with clinical disease activity in patients with MCTD.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00267336

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Determination of the immunosuppressive drug tacrolimus in its dosage forms by high-performance liquid chromatography (1995)

Namiki, Y. ; Fujiwara, A. ; Kihara, N. ; [et al.]

Springer

Chromatographia 40 (1995), S. 253-258

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ISSN: 1612-1112

Keywords: Column Liquid Chromatography ; Immunosuppessive drug FK 506 (Tacrolimus) ; Tautomeric Phenomenon

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary A high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of FK506, a new immunosuppressant, in bulk drug samples and dosage forms. Equilibration between FK506 (I) and its tautomeric compounds (II and III) was accomplished “artificially” in water-dehydrated alcohol (1∶1) employed as an extraction solvent. After reaching equilibrium, separation of I, treated as the representetive of equilibrated FK506, from its related substances was achieved by reversed-phase HPLC on a C18 column with water-isopropyl alcohol-tetrahydrofuran (5∶2∶2, v/v) as the mobile phase, and detection at 220 nm. Component I in dosage forms could be recovered satisfactorily and determined with good precision. The calibration graph was linear over the range 2–6 μg for I.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02290354

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