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Prevention of Death of Axotomized Hypoglossal Neurones and Promotion of Regeneration by Chitin Grafting (2000)

Itoh, Michi-Ichirou ; Izumi, Shin-Ichi ; Uemura, Masanori ; [et al.]

Springer

Cellular and molecular neurobiology 20 (2000), S. 529-540

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ISSN: 1573-6830

Keywords: chitin ; biomaterials ; nerve regeneration ; hypoglossal nerve ; shrew

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract 1. Chitin is known to promote skin wound healing. In this study, chitin, prepared from Zuwai crab shell, was used as a bridge between the proximal and distal stumps of cut hypoglossal nerves in shrews. We compared the effects of chitin on the regeneration of transected right hypoglossal nerve axons, with those of porcine dermis, bovine dermal aterocollagen, and autologous nerve bundles. 2. To assess the survival of neurones, the size of neuronal cell body, and number of motoneurones were determined in the absence of any bridged material and in the presence of porcine dermis, bovine dermal aterocollagen, chitin, or autologous nerve bundles as a bridge. 3. Our results revealed a significantly better outcome in chitin and autologous nerve bridged groups; the size of neuronal cell body and number of hypoglossal neurones were higher than in the other groups. Chitin also enhanced the regeneration of neurones; the number of horseradish peroxide positive neurones indicative of repaired axonal processes was significantly higher in chitin and autologous nerve-bridged groups than in other groups. 4. Our results demonstrated that the use of chitin sheet or autograft successfully prevented the death of severed neurones and promoted the regeneration of the lesioned nerve. Although the mechanisms underlying the effects of chitin are still unknown, chitin seems to be a potentially useful biocompatible material for nerve repair and regeneration.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007055626632

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In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: Specific expression in germ cells at stages around meiotic cell division (1992)

Koji, Takehiko ; Jinno, Atsushi ; Matsushime, Hitoshi ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 10 (1992), S. 273-279

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ISSN: 0263-6484

Keywords: In situ hybridization ; v-ros tyrosine kinase ; male germ cell-associated kinase ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Biochemical analysis of the male germ cell-associated kinase (mak) gene, which was isolated recently by using weak cross-hybridization with the v-ros tyrosine kinase gene, revealed that the gene was highly expressed in mammalian testicular germ cells, but not in ovarian cells. In order to identify the cells which express the mak gene in more detail, we localized mak mRNA in frozen sections of mouse testis by non-radioactive in situ hybridization. In this study, we utilized thymine-thymine (T-T) dimerized mak cDNA as a haptenic, non-radioactive probe, and the signal was detected enzyme-immunohistochemically by using an anti-T-T antibody. As a result, mak mRNA was localized intensely in late pachytene (stage X) and diplotene (stage XI) spermatocytes, and faintly in dividing spermatocytes (stage XII) and early round spermatids (stage I-II), suggesting that the gene may play an important role in the phase around meiotic cell division, but not throughout the entire meiosis.

Additional Material: 2 Ill.