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  • Internalization  (1)
  • Key words Immunotoxin  (1)
  • L-phenylalanine Inhibition  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Different cytotoxic activity and intracellular fate of an anti-CD5–momordin immunotoxin in normal compared to tumour cells (1995)
    Springer
    Cancer immunology immunotherapy 40 (1995), S. 213-218 
    Details
    ISSN: 1432-0851
    Keywords: Key words Immunotoxin ; CD5 ; Momordin ; Internalization ; Cytotoxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We investigated the different sensitivity of peripheral blood mononuclear cells (PBMC) and human T cell leukaemias (Jurkat and CEM) to an anti-CD5–momordin immunotoxin. In a short-term assay, the immunotoxin displayed different cytotoxic activity on normal and tumour cells: for leukaemic cell lines an incubation time of 72 h was necessary for the immunotoxin to reach the IC50 of 41–53 pM, compared to the 1 h sufficient for 6 pM immunotoxin to inhibit 50% of PBMC protein synthesis. In a long-term clonogenic assay (15 days), the immunotoxin demonstrated a comparable efficacy of clonogenic cell killing for both cell types. We investigated the immunotoxin internalization pathway by a flow-cytometric method and our data seem to indicate that the molecules meet a different intracellular fate in the two cell populations. It may be assumed that the low cytotoxic activity of immunotoxins on tumour cells, detected in the short-term assay, is due to inefficient delivery to their cytoplasmatic target, while a longer exposure of the cells to the immunotoxin promotes adequate intracellular distribution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01519894
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of serum alkaline phosphatase in infancy and childhood (1972)
    Springer
    European journal of pediatrics 113 (1972), S. 289-296 
    Details
    ISSN: 1432-1076
    Keywords: Serum Alkaline Phosphatase ; Acrylamide Gel Disc Electrophoresis ; L-phenylalanine Inhibition ; L-Homoarginine Inhibition ; Neuraminidase Sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In 364 Seren von Neugeborenen (Nabelschnurblut), Säuglingen, Kindern und Erwachsenen wurde die vorhandene alkalische Phosphatase genauer differenziert unter Anwendung der L-Phenylalaninhemmung, der L-Homoargininhemmung, der Hitzedenaturierung, der Arcylamidgel-Scheibenelektrophorese und der Neuramidaseempfindlichkeit. Die hohen Werte der alkalischen Phosphatase im Nabelschnur-, Säuglings-und Kinderserum im Vergleich zu den Erwachsenenwerten werden mehr den Phosphatasen der Knochen als den intestinalen Phosphatasen zugerechnet. Mit Hilfe der Acrylgelelektrophorese konnte eine Zunahme der intestinalen alkalischen Phosphatase mit steigendem Lebensalter nachgewiesen werden.
    Notes: Abstract In 364 serum samples of cord blood, infants, children, and adults alkaline phosphatase was characterized by means of L-phenylalanine inhibition, L-homoarginine inhibition, heat inactivation, acrylamide gel disc electrophoresis, and neuraminidase sensitivity. The increase of serum alkaline phosphatase in cord blood serum and in infant's and children's serum in comparison with adult levels was attributed mainly to the bone enzyme and in smaller proportion to the intestinal enzyme. Using acrylamide gel disc electrophoresis, an increase in the frequency of subjects with an intestinal alkaline phosphatase band with increasing age values was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440307
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    Paper (German National Licenses)
    Fulltext
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