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  • 1
    Electronic Resource
    Electronic Resource
    Invasiveness of human glioma cell lines in vitro: Relation to tumorigenicity in athymic mice (1987)
    Springer
    Acta neuropathologica 72 (1987), S. 207-213 
    Details
    ISSN: 1432-0533
    Keywords: Human glioblastomas ; Invasion ; Organ culture ; Tumorigenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five established cell lines derived from human anaplastic astrocytomas or glioblastoma multiforme were tested for invasiveness into precultured chick heart fragments in vitro. Four of the cell lines (U118 MG, D54 MG, U373 MG and A172) were strongly invasive into the heart tissue. A fifth cell line, U251 MG sp, which was only tumorigenic at doses of greater than 1×108 cells in athymic mice, was non-invasive in vitro. One line, A172, was invasive but not tumorigenic in athymic mice, although a related invasive subline, D54 MG, at later passage levels was tumorigenic even at low cell doses. Invasion of the glioma cells was characterized by progressive and irreversible replacement of the precultured chick heart tissue. Both by light and transmission electron microscopy, a similar pattern of invasion was observed as earlier found with experimental rat glioma cells in the same system. Some human cell lines established from human gliomas retain invasive properties after a prolonged culture period in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691091
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of growth factors on a human glioma cell line during invasion into rat brain aggregates in culture (1992)
    Springer
    Acta neuropathologica 84 (1992), S. 190-197 
    Details
    ISSN: 1432-0533
    Keywords: Brain neoplasm ; Culture ; Invasion ; Growth factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cultures of fetal rat brain cell aggregates and tumor spheroids from the human glioma cell line GaMG were treated with epidermal growth factor (EGF), fibroblast growth factor (FGF) or isoforms of platelet-derived growth factor (PDGF AA or BB). Radioreceptor binding studies displayed a high binding capacity for EGF and FGF, but not binding of PDGF isoforms in the glioma cells. In serum-free culture, 10 ng/ml of both EGF and FGF caused increased growth and cell shedding in the tumor spheroids, whereas PDGF produced no such effect. Similarly, EGF and FGF stimulated tumor cell migration. EGF increased the proliferation and outgrowth of glial fibrillary acidic protein (GFAP)-positive cells in brain cell aggregates, while PDGF AA and BB both stimulated the outgrowth of oligodendrocyte-like cells which were negative for GFAP and neuron-specific enolase. FGF stimulated GFAP+ as well as GFAP− cell types. In co-culture experiments using brain aggregates and tumor spheroids, both EGF and FGF treatment caused increased tumor cell invasion. PDGF had no effect on the tumor cells, but instead stimulated the proliferation of oligodendrocyte-like cells in the brain aggregates. The present results indicate that growth factors may facilitate glioma growth as well as invasiveness, and cause reactive changes in the surrounding normal tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00311394
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Interaction between human medulloblastomas and foetal rat brain aggregates in vitro (1994)
    Springer
    Acta neurochirurgica 126 (1994), S. 11-16 
    Details
    ISSN: 0942-0940
    Keywords: Medulloblastoma ; rhabdomyosarcoma ; organ co-culture assay system ; brain tissue invasion ; in vitro investigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The invasiveness of three medulloblastoma permanent cell lines (D-283, D-341, and DAOY), a human medulloblastoma biopsy, and in addition, a human rhabdomyosarcoma permanent cell line (TE-671), which previously had been regarded as a human medulloblastoma, was studied in an organ co-culture assay. All the four cell lines and the biopsy were co-cultured with normal rat brain cell aggregates for up to six days in vitro. The medulloblastoma biopsy, the D-283 and the D-341 cells invaded the brain tissue by diffuse single cell infiltration. The medulloblastoma cell line (DAOY) showed an invasive pattern similar to that observed earlier for most glioblastoma cell lines. This was characterized by massive cell replacement and destruction of normal brain tissue. The rhabdomyosarcoma cell line (TE-671) presented a solid invasive pattern with a fairly well defined border between normal brain and tumour tissue. Thus, the organ co-culture assay system in vitro seems to mimic several aspects of the in situ invasive behaviour of medulloblastomas. It may, therefore, provide new perspectives for pretreatment investigations with chemotherapy and radiotherapy of these malignancies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476488
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    Paper (German National Licenses)
    Fulltext
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