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  • Isoelectric focusing pattern  (1)
  • 1
    ISSN: 1573-5028
    Keywords: Nicotiana ; Isoelectric focusing pattern ; rbcL gene ; sequence homologies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Comparison of the DNA sequences of the rbcL gene from three Nicotiana species reveals a high degree of homology among the 1431 bp in the coding region. Only eight base pair differences are observed between N. otophora and N. tabacum, and between N. otophora and N. acuminata. Four base pair differences are observed between N. acuminata and N. tabacum. Most changes are in the third position of the codon resulting in only two amino acid alterations when N. otophora and N. acuminata are compared with N. tabacum. Evidence is presented demonstrating that the amino acid compositions of the LS derived from the DNA sequence are related to the IEF cluster pattern. A single charged residue is responsible for the difference in cluster pattern.
    Type of Medium: Electronic Resource
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