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  • 1
    Electronic Resource
    Electronic Resource
    Inhibitory effects of 4,4′-diisothiocyano stilbene-2,2′-disulfonic acid (DIDS) in the response of isolated hepatocytes to phalloidin (1978)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 304 (1978), S. 303-307 
    Details
    ISSN: 1432-1912
    Keywords: Phalloidin poisoning ; Isolated hepatocytes ; 4,4′-diisothiocyano stilbene-2,2′-disulfonic acid ; Plasma membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 4,4′-Diisothiocyano stilbene-2,2′-disulfonic acid (DIDS) inhibits the typical development of protrusions, regularly seen after treatment of isolated hepatocytes with phalloidin. The degree of inhibition depends on the time of preincubation and on the concentration of DIDS, but not on the concentration of phalloidin. DIDS is more effective than H2DIDS. The inhibition by both compounds is irreversible. The binding capacity of hepatocytes for H2DIDS is much higher than that of the phalloidin-insensitive hepatoma cells. Gel electrophoresis of lysates from cells, pretreated with 3H2DIDS demonstrates that actin binds very little of the inhibitor. Our results suggest that a protein structure on the surface of hepatocytes, needed for the response to phalloidin, is influenced by DIDS or H2DIDS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00507973
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Affinity labels for membrane components involved in the uptake of bile acids and of phallotoxins by hepatocytes (1982)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 319 (1982), S. 249-253 
    Details
    ISSN: 1432-1912
    Keywords: Isolated hepatocytes ; Phallotoxin uptake ; Bile salt transport ; Covalently coupling derivatives of bile salts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of covalently binding derivatives of bile acids, fusidic acid and of compounds similar to cholecystographic agents were synthesized. Nearly all of them inhibited the development of protrusions on the surface of isolated hepatocytes regularly seen after treatment with phalloidin. The same compounds inhibited the uptake of demethylphalloin and of cholate in a concentration dependent manner. Two kinds of effects could be distinguished: The irreversible part of the inhibition depended on the incubation period and could not be removed by washing procedures. The reversible one was independent on the duration of the preincubation. Final results indicated that the tested derivatives inhibited either both transports, and the phalloidin response of liver cells to the same degree and in the same manner, or were found to be ineffective in all tests. The above parallelism supports the hypothesis that phallotoxins may be translocated by a carrier system normally responsible for the uptake of bile acids from the portal blood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495874
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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