ISSN:
1573-5044
Keywords:
Lactuca sativa
;
regeneration
;
tissue culture
;
transgenic plant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transgenic plants of lettuce cultivar (cv.) ‘South Bay’ were produced by using Agrobacterium tumefaciens vectors containing the β-glucuronidase (GUS) reporter gene and the NPT II gene for kanamycin resistance as a selectable marker. High frequency of transformation, based on kanamycin resistance and assays for GUS expression, was obtained with 24 to 72-h-old cotyledon explants cocultivated for 48 h with Agrobacterium tumefaciens. After the cocultivation period, the explants were placed in selection medium containing 50 or 100 mg l−1 of kanamycin, 100 mg l−1 cefotaxime and 500 mg l−1 carbenicillin for 10 days. Surviving explants were transferred every 14 days on shoot elongation medium. Progenies of R0 plants demonstrated linked monogenic segregation for kanamycin resistance and GUS activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00029717
Permalink