ISSN:
1435-1536
Keywords:
Emulsion polymerization
;
latex
;
microsphere
;
enzyme
;
immobilization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract Effects of the surface hydrophilicity of styrene-2-hydroxyethyl methacrylate (S-HEMA) copolymer microspheres on the adsorption immobilization of trypsin and on its enzymatic activity were discussed. In the case of a polystyrene (PS) microsphere, the amount of adsorption was large, whereas the enzymatic activity of the adsorbed trypsin was low. On the other hand, in the case of an S-HEMA copolymer microsphere whose HEMA content was 10 mole %, the maximum specific activity of trypsin adsorbed was nearly equal to that of free trypsin, although the amount of adsorption decreased to about 65 % of that in PS. Accordingly, total activity, which is the product of the amount of adsorption and the specific activity, could be enhanced by controlling the surface hydrophilicity of the microsphere.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01500748
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