ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
By using particle electrophoresis and quantitative analysis the interaction of anionic surfactants (sodium dodecylculfate, sodium decylbenzene sulfonate) with a hydrocarbon-grown yeast and a commercial baker's yeast has been investigated to obtain further informations about the chemical composition of the surface region (3-6 nm) of the cell wall of this yeasts. A correlation is found between the chemical composition and the different adsorption behaviour of various batches of the same yeasts. It was found that surface-localized hydrophobic glycoproteins, probably proteophosphomannan are responsible for the strong pH dependent adsorption behaviour of a typical Candida species.In contrast the cell surface of a typical Saccharomyces species was strongly hydrophilic and showed no surfactant adsorption.This can be explained by the presence of a polysaccharide probably phosphomannan in the surface region only.The implication of proteins in the adsorption process is confirmed by model experiments too.The models for the yeast cell wall proposed by LAMPEN and KIDBY et al. were critically discussed in terms of our results.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370060305
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