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A single amino-acid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity (1992)

Fujimura, Makoto ; Oeda, Kenji ; Inoue, Hirokazu ; [et al.]

Springer

Current genetics 21 (1992), S. 399-404

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ISSN: 1432-0983

Keywords: Neurospora ; Beta-tubulin ; Benzimidazoles ; N-phenylcarbamates

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Two MBC-resistant mutants of Neurospora crassa, F914 and F939, were sensitive to diethofencarb at a concentration of 0.1 μg/ml, while the wild-type strain and other MBC-resistant mutants showed resistance to diethofencarb at a concentration of 100 μg/ml. Genetic analysis suggested that the mutations in these two strains were closely linked to the Bml locus which codes for beta-tubulin. When the wild-type strain was transformed by the cloned beta-tubulin gene of the F914 strain, the transformants showed both MBC resistance and diethofencarb sensitivity. On the other hand, the diethofencarb sensitivity of the F914 strain was cancelled by transformation with the wild-type beta-tubulin gene. DNA sequencing of F914 beta-tubulin revealed that glycine was substituted for glutamic acid at position 198 in the F914 strain. Therefore, a single base change in the betatubulin gene was proved to confer both MBC resistance and diethofencarb sensitivity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00351701

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Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV) (1990)

Oka, Takashi ; Yutsudo, Masuo ; Inoue, Hirokazu ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 145 (1990), S. 428-433

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. Molecular Biological analysis of thre revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. These findings suggest the existence of a specific mechanism(s) for elimination of the v-abl oncogene by segregation, mutation, or gene rearrangement in these cells.

Additional Material: 5 Ill.