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  • 1
    ISSN: 1573-5036
    Keywords: Dolichos ; Greenhouse ; Growth ; Lablab ; Lime ; Nodulation ; Phosphorus requirement ; Rhizobium strain ; Soil acidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Greenhouse experiments were done with two purposes: (1) to identify strains of rhizobia effective and acid-tolerant in symbiosis withLablab purpureus, and (2) to determine whether soil acidity or the symbiotic condition increased the phosphate requirement for growth. Five rhizobial strains were tested in one neutral soil, two acid soils, and the two acid soils limed to pH 6.6. In the neutral and limed soils, three of the strains were effective (CB1024, CB756, TAL169), but only two strains (CB756, TAL169) remained effective in acid soil. Strain CB756 and plus-N treatments were further compared in a factorial trial involving combinations of five levels of P with lime, no lime and CaCl2 treatments, applied to an acid soil. Some of the treatments were also applied to plants inoculated with CB1024. Between the N-fertilized and CB756 treatments there was no clear difference in growth response to applied P, and the critical internal concentration of P for 95% of maximal growth was the same (0.22% shoot dry weight). Increasing P beyond levels needed for maximal growth increased nodulation and N concentration in plants inoculated with CB756. It lowered N concentration in N-fertilized plants. There was evidence suggesting that the P requirement of symbiotic plants increased if the soil was acid, or if CB756 were replaced by CB1024 as microsymbiont; but the critical statistical interactions were not significant.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5036
    Keywords: Glycine ; Greenhouse ; N accumulation ; Nodulation ; Ontogeny ; Phaseolus ; Rhizobia ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Greenhouse experiments were done under favorable conditions to compare effective bean symbioses with cowpea and soybean symbioses and with N-fertilized controls. Growth, N-accumulation, nodulation, acetylene reduction, hydrogen evolution and the effect of native rhizobia on symbiotic performance were evaluated. Relative N accumulation (in symbiotic plants relative to N-fertilized plants) was higher for soybean (43%) than for the other symbioses (25–39%) at four weeks, but from four to six weeks both soybean (96%) and cowpea (92%) accumulated relatively more N than did beans (56–78%). Inferior performance of beans could not be atributed to differences in acetylene reduction or nodule weight, but bean nodules were smaller and more numerous and evolved more hydrogen (Relative energetic efficiency was 0.5 to 0.7 in bean, 0.95 in cowpea and soybean). Relative N accumulation was influenced by N accumulation characteristics of the fertilized plants as well as the symbiotic plants. The vegetative N-fixation period of early maturing beans was shorter than for cowpeas of similar maturation date; the beans flowered earlier and had a longer pod-filling period. There was no evidence that the common bean symbiosis was more sensitive than the others to competition from native rhizobia. With mixed populations of effective rhizobia, hydrogen evolution in otherPhaseolus species (P. acutifolius, P. coccineus, P. filiformis, P. lunatus) was similar to that inP. vulgaris and higher than in cowpea or soybean. Although failure to establish effective nodulation is often considered the reason for poor N-fixation by common bean in the field, the species may be genetically predisposed to poor fixation because of symbiotic inefficiency and the short vegetative fixation period.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 98 (1987), S. 183-194 
    ISSN: 1573-5036
    Keywords: Glycine ; Mulching ; N fixation ; Nodulation ; Phaseolus ; Rhizobium Soil ; Temperature ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experiments were done to test whether N fixation is more sensitive to high soil temperatures in common bean than in cowpea or soybean. Greenhouse experiments compared nodulation, nitrogenase activity, growth and nitrogen accumulation of several host/strain combinations of common bean with the other grain legumes and with N-fertilization, at various root temperatures. Field experiments compared relative N-accumulation (in symbiotic relative to N-fertilized plants) of common bean with cowpea under different soil thermal regimes. N-fertilized beans were unaffected by the higher temperatures, but nitrogen accumulation by symbiotic beans was always more sensitive to high root temperatures (33°C, 33/28°C, 34/28°C compared with 28°C) than were cowpea and soybean symbiosis. Healthy bean nodules that had developed at low temperatures functioned normally in acetylene reduction tests done at 35°C. High temperatures caused little or no suppression of nodule number. However, bean nodules produced at high temperatures were small and had low specific activity. ForP. vulgaris some tolerance to high temperature was observed among rhizobium strains (e.g., CIAT 899 was tolerant) but not among host cultivars. Heat tolerance ofP. acutifolius andP. lunatus symbioses was similar to that of cowpea and soybean. In the field, high surface soil temperatures did not reduce N accumulation in symbiotic beans more than in cowpea, probably because of compensatory nodulation in the deeper and cooler parts of the soil.
    Type of Medium: Electronic Resource
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