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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts (1987)
    Springer
    Archives of microbiology 146 (1987), S. 353-357 
    Details
    ISSN: 1432-072X
    Keywords: Protoplast ; Plasmid ; Transformation ; Bacillus subtilis ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed [4.0×107 transformants per μg DNA (one transformant per 5×104 molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410935
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