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  • Recombination  (2)
  • Linkage mapping  (1)
  • Maize cytoplasmic male sterility  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Variation in the ratio of physical to genetic distance in intervals adjacent to theMla locus on barley chromosome 1H (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 472-482 
    Details
    ISSN: 1617-4623
    Keywords: Barley ; Physical mapping ; Recombination ; Powdery mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H.2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kbMluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted withNotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in theXbcd249.2-Xmwg068 interval and 0.465 Mb/cM in theXbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate theXbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than theXbcd249.2-Xmwg068 interval.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172376
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Mapping complementary genes in maize: positioning the rf1 and rf2 nuclear-fertility restorer loci of Texas (T) cytoplasm relative to RFLP and visible markers (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 785-795 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Cytoplasmic male sterility ; Fertility restorer loci ; Linkage mapping ; Complementary genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There are three major groups of cytoplasmic male-sterile cytoplasms in maize; C (Charrua), S (USDA), and T (Texas). These cytoplasms can be classified by the unique nuclear genes that suppress the male-sterility effects of these cytoplasms and restore pollen fertility. Typically, plants that carry Texas (T) cytoplasm are male fertile only if they carry dominant alleles at two unlinked nuclear restorer loci,rf1 andrf2. To facilitate analysis of T-cytoplasm-mediated male sterility and fertility restoration, we have mappedrf1 andrf2 relative to closely-linked RFLP markers using five populations. Therf1 locus and/or linked visible markers were mapped in four populations; therf2 locus was mapped in two of the populations. Data from the individual populations were joined with the aid of JoinMap software. The resulting consensus maps placerf1 between umc97 and umc92 on chromosome 3 andrf2 between umc153 andsus1 on chromosome 9. Markers that flank therf1 andrf2 loci have been used to identify alleles atrf1 andrf2 in segregating populations. These analyses demonstrate the possibility of tracking separate fertility restorer loci that contribute to a single phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253987
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Expression in Saccharomyces cerevisiae of a gene associated with cytoplasmic male sterility from maize: Respiratory dysfunction and uncoupling of yeast mitochondria (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 24-32 
    Details
    ISSN: 1617-4623
    Keywords: Cochliobolus heterostrophus race T disease ; Maize cytoplasmic male sterility ; Mitochondria ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We asked whether the mitochondrial T-urf13 gene, associated with the male sterility phenotype of T cytoplasm in maize, can be expressed in Saccharomyces cerevisiae and whether this expression can mimic the effects observed in maize. We introduced the universal code equivalent of the T-urf13 gene into the S. cerevisiae nucleus by transformation and directed its translation product into mitochondria by means of a fusion with the targeting presequence from Neurospora crassa ATPase subunit 9. We show that expression of the universal code equivalent of the T-urf13 gene in the yeast nucleus does indeed mimic its effects in maize: respiratory growth of yeast is inhibited, respiration-deficient cytoplasmic mutants accumulate and NADH oxidation of isolated mitochondria is uncoupled. All these effects are observed only if the mitochondrial targeting peptide and methomyl or HmT toxin are present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315793
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla  locus on barley chromosome 1H (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 472-482 
    Details
    ISSN: 1617-4623
    Keywords: Key words Barley ; Physical mapping ; Recombination ; Powdery mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172376
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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