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  • 1
    Electronic Resource
    Electronic Resource
    Modulation of granulocyte oxidative response by recombinant interferon α2 and γ (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 274-276 
    Details
    ISSN: 1432-069X
    Keywords: Interferon α2 ; Interferon γ ; Granulocytes ; Chemiluminescence ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interferon (IFN) has been described to influence various cellular functions. In this study we investigated whether the oxidative response of polymorphonuclear leukocytes (PMN) is also affected by IFN. In order to exclude the possible influence of impurities in IFN preparations, only recombinant human IFN α2 or γ were used. Lucigenin-dependent chemiluminescence (CL) of PMN was measured to assess the production of oxygen radicals. IFN γ at a concentration of more than 10 ng/ml elicited a minimal CL response in PMN. When PMN were incubated with IFN γ for 1 h and then stimulated with chemotactic peptide f-met-phe (FMP), zymosan-activated serum (ZAS), zymosan particles, or phorbol-myristate acetate (PMA), the CL response was increased as consequence of the generally enhanced oxidative metabolism. IFN α2 showed no such effect at any concentration tested. A 5-min pretreatment with IFN γ decreased the ZAS response but did not affect the reaction to the other stimuli. The possibility of a generation of IFN by PMN during the assay could be excluded as no IFN activity could be detected in an antiviral assay after stimulation of PMN for 6 h with PolyIxPolyC, LPS, ConA, C. parvum, PMA, zymosan, or FMP. The modulation of granulocyte activity by IFN γ may be important in the regulation of the anti-inflammatory response of PMN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407737
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of complement activation in human serum using C5S-Induced chemiluminescence of human granulocytes (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 41-43 
    Details
    ISSN: 1432-069X
    Keywords: Complement ; Anaphylatoxins ; C5a ; Chemiluminescence ; Granulocytes ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The complement system can be activated by many factors, including immune complexes, leading to the generation of biologically active split products like C5a anaphylatoxin. This study presents a technique which may be used for measuring C5a activity in human serum. The tetanus-anti-tetanus immune complex and aggregated human IgG were used as model activators of the complement cascade. The C5a activity was measured by C5a-induced chemiluminescence of granulocytes; furthermore, a radioimmunoassay was used to detect the C5a peptide. There was a strongly positive correlation between the two assay systems. The described method should be useful as an alternative means of detecting complement activation in the serum of patients with inflammatory diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412494
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Involvement of complement in psoriasis and atopic dermatitis — Measurement of C3a and C5a, C3, C4 and C1 inactivator (1985)
    Springer
    Archives of dermatological research 277 (1985), S. 359-361 
    Details
    ISSN: 1432-069X
    Keywords: Complement ; Anaphylatoxins ; Psoriasis ; Atopic dermatitis ; C3a ; C5a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Normal complement components and activation products were determined in the peripheral blood of 35 patients with atopic dermatitis (AD) and 24 patients with psoriasis at a mild to intermediate stage. None of the patients had received systemic or local steroid therapy 6 weeks prior to blood collection. Levels of C3, C4 and C1 inactivator (C1 INA) were determined in serum by radial immunodiffusion, whereas C3a and C5a levels were measured by radioimmunoassay. In comparison to healthy non-atopic controls, the levels of C3, C4 and C1 INA were found to be significantly increased in both diseases. No substantial differences were detected between patients with psoriasis vulgaris and psoriasis guttata, which suggests that the dissimilarities found were not due to preceding or concomitant infections. In AD, there was a tendency towards increased C3a levels, whereas in psoriasis, C3a levels were significantly increased. In both diseases, no measurable amounts of C5a could be detected. The results indicate that, in both AD and psoriasis, the complement participates in the inflammatory process. Elevated levels of C3a suggest that there is a continuous activation of the complement system leading to the generation of inflammatory mediators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00509233
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Elevated levels of soluble CD8 antigen in sera of patients with psoriasis — a possible sign of suppressor/cytotoxic T-cell activation (1990)
    Springer
    Archives of dermatological research 282 (1990), S. 6-7 
    Details
    ISSN: 1432-069X
    Keywords: Psoriasis ; T-cell activation ; CD8 antigen ; Psoriatic arthritis ; Atopic dermatitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Increased levels of the soluble CD8 antigen probably represent a sign of suppressor T-cell activity. The concentration of CD8 was measured in the sera of patients with psoriasis without psoriatic arthritis (n=25) and atopic dermatitis (n=26), exhibiting moderate to severe disease activity, in comparison with nonatopic healthy controls (n=31) using an ELISA technique. CD8 levels were found to be significantly elevated in psoriatic patients as compared with healthy controls (p≤0.002). Increase in CD8 may be due to suppressor T-cell activation in the skin of patients with psoriasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00505637
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    Paper (German National Licenses)
    Fulltext
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