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  • 1
    Electronic Resource
    Electronic Resource
    Photosynthetically active suspension cultures of potato spindle tuber viroid infected tomato cells as tools for studying viroid — host cell interaction (1993)
    Springer
    Plant cell reports 12 (1993), S. 597-602 
    Details
    ISSN: 1432-203X
    Keywords: Lycopersicon peruvianum ; Lycopersicon esculentum ; viroid pathogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media containing 3% sucrose, but during continuous photo-mixotrophic culture in low sucrose media (1% sucrose), the level of PSTVd accumulation decreased. Photoautotrophic cell suspensions could be established with uninfected, but not with viroid infected tomato cells. As compared to uninfected cells, PSTVd infected cells grew slowly, were morphologically different in size and shape, and formed tight cell aggregates. Electronmicroscopy showed that starch accumulation in chloroplasts, deformation of the chloroplast envelope and irregular plasmalemmasomes at the cell membrane were associated with PSTVd infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232806
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Response to chilling of tomato mesophyll protoplasts (1981)
    Springer
    Planta 151 (1981), S. 399-401 
    Details
    ISSN: 1432-2048
    Keywords: Chilling effect ; Lycopersicon ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Freshly isolated protoplasts from tomato leaves show two completely different responses to a chilling treatment of 12 h at 7° C prior to culture at 29° C, depending on the presence or absence of glucose in the medium. In the culture medium with glucose as osmoticum, where the rate of cell divisions under optimal culture conditions is relatively high (about 20% plating efficiency), protoplasts were drastically injured by the chilling procedure and died. In the medium with mannitol as the osmoticum instead of glucose, where the plating efficiency even under optimal conditions is rather low (about 8%), protoplasts withstand the chilling procedure. More-over, after the chilling treatment when the protoplasts were transferred to the optimal culture temperature of 29° C, the plating efficiency was raised to about 20%, which is the same level as in the glucose-containing medium without chilling. This effect was not observed when the medium in which the protoplasts were suspended during the chilling period was replaced with fresh medium. This suggests that under these conditions tomato protoplasts produce and excrete a factor in the cold that improves the vitality of the cells or stimulates cell division. The possible relationship between chilling sensitivity of tomato protoplasts and their ability to divide will be discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393298
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Different regeneration potential of mesophyll protoplasts from cultivated and a wild species of tomato (1980)
    Springer
    Planta 148 (1980), S. 89-96 
    Details
    ISSN: 1432-2048
    Keywords: Lycopersicon ; Protoplasts ; Regeneration (roots, shorts)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mesophyll protoplasts were isolated from leaves of three cultivars of Lycopersicon esculentum (L.) Mill., namely “Hilda 72”, “Rutgers” and “Rentita”, and from the wild tomato species Lycopersicon peruvianum (L.) Mill. Protoplasts from L. peruvianum divided and grew actively in a liquid medium according to Zapata et al. (1977), whereas protoplasts from the tomato cultivars “Hilda 72” and “Rutgers” showed comparable rates for cell division only, when the content of major elements in this medium was reduced to one half of the original concentration and when mannitol as osmoticum was replaced by glucose. In “Rentita” protoplasts no cell division could be observed in about 15 different modifications of the five basic culture media tested. The morphogenetic potential of these tomato cells was assessed by comparing the root and shoot formation of protoplasts and of leaf explants. L. peruvianum exhibited the highest potential. Calli derived from protoplasts regenerated roots on Murastrige-Skoog agar containing 1 μM benzylaminopurine (BAP) plus 10 μM indole-3-acetic acid (IAA) and 0.1 μM BAP plus 1 μM IAA. Shoot formation occurred in the combinations of 10 μM BAP with 0.1, 1.0, and 10 μM IAA. Plantlets regenerated from the L. peruvianum calli could be grown in soil. No shoots or roots were regenerated from calli of “Hilda 72” and “Rutgers” protoplasts in all combinations of BAP and IAA tested in the range from 0.1 μM to 100 μM, thus indicating the rather low morphogenetic potential of these protoplasts as compared to protoplasts from L. peruvianum leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385447
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    Paper (German National Licenses)
    Fulltext
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