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  • 1
    ISSN: 1432-0584
    Keywords: Lymphozyten-Aktivierung ; menschliche Lymphozyten-Subpopulationen ; Monozytenfunktion ; Lymphocyte activation ; Human lymphocyte subpopulations ; Monocyte function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Cowan I strain Staphylococcus aureus bacteria were found to be mitogenic for human peripheral and cord blood lymphocytes. Experiments with lymphocyte subpopulations obtained by nylon wool filtration and/or E-rosette separation revealed that T-lymphocytes are the main target cells, whereas isolated B cells did not respond significantly. Further experiments suggested that B cells could be activated in the presence of mitomycin-treated T cells. Null cell-enriched lymphocyte suspensions could be stimulated by Con A but not by the bacteria or by PHA.
    Notes: Zusammenfassung Staphylococcus aureus-Bakterien des Stammes Cowan I sind in der Lage, periphere menschliche Lymphozyten sowie Lymphozyten des Nabelvenenblutes in vitro zu stimulieren. Versuche mit Lymphozyten-Subpopuiationen, die mit Hilfe der Nylonsäulen-Trennung und/oder der E-RosettenTrennung gewonnen wurden, haben gezeigt, daß in erster Linie T-Lymphozyten aktiviert werden; isolierte B-Zellen wurden nicht stimuliert. Weitere Experimente lassen jedoch vermuten, daß B-Lymphozyten dann aktiviert werden, wenn Mitomycin-behandelte T-Zellen in den Kulturen vorhanden sind. Lymphozyten-Subpopulationen, die angereichert Null-Zellen enthielten, konnten zwar durch Con A, nicht aber durch die Bakterien oder durch PHA stimuliert werden.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 288 (1975), S. 7-16 
    ISSN: 1432-1912
    Keywords: Hydroxyurea ; Mammalian Embryo ; Nucleic Acid ; in vivo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 14C-u-glucose and 32P-phosphate were given intravenously to pregnant rats, treated and untreated with hydroxyurea (HU). The incorporation of radio-activity into a variety of cell components of the embryo was measured. With this double-labelling technique it is possible to survey the effects of embryotoxic drugs on various pathways in mammalian embryos in vivo. Onset, extent and duration of the metabolic changes were measured after application of “non-teratogenic” and teratogenic doses of hydroxyurea (HU). 3 hrs after application of hydroxyurea only DNA synthesis is affected, whereas after 5 hrs RNA synthesis is also inhibited.
    Type of Medium: Electronic Resource
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