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  • 1
    Electronic Resource
    Electronic Resource
    A Non-Ionic Vesicle Lipid Enhances Mastoparan-Stimulated GTPase Activity of Heterotrimeric G-Proteins (1995)
    Springer
    Pharmaceutical research 12 (1995), S. 366-369 
    Details
    ISSN: 1573-904X
    Keywords: G-protein ; Mastoparan ; liposomes ; Macrogol-260-cetylstearylether ; reconstitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Isolated heterotrimeric G-proteins exhibit full biological activity when reconstituted into liposomes. Here, we investigated the non-ionic surfactant macrogol-260-cetylstearylether (TA 6) as an efficient vehicle for the reconstitution of G-proteins. Reconstitution efficiency of G-proteins was recorded by GTPγS-binding. Their biological potency was measured as basal and mastoparan-stimulated GTPase-activity. G-proteins were fully active when associated with liposomes. On the other hand, G-proteins solubilized by TA 6 micelles or reconstituted into pure TA 6 vesicles exhibited impaired biological activity. However, vesicles containing different ratios of azolectin and non-ionic TA 6 showed about 50% higher reconstitution efficiency as compared to pure liposomes. In addition, basal and mastoparan-stimulated GTPase-activity in vesicles containing an axolectin / TA 6 ratio of 3:1 were 2.7 and 9.1 fold higher than in pure liposomes, respectively. These data emphasize that the composition of the lipid membranous environment significantly influences G-protein activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1016296300654
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Über α-Hydroxyalkyl-acyl-sulfide und α-Halogenalkyl-acyl-sulfide (1959)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 623 (1959), S. 92-102 
    Details
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Thiocarbonsäuren bilden mit Aldehyden relativ beständige Halbmercaptale, R - CH(OH) - S - CO - R', deren Eigenschaften beschrieben werden. Durch Umsetzung mit anorganischen Säurechloriden entstehen daraus α-Halogenalkyl-acyl-sulfide, R - CH(Cl) - S - CO - R', die durch ein sehr reaktionsfähiges Halogen ausgezeichnet sind und sich zur Einführung von Mercaptoalkylgruppen eignen.  -  Die Hydrolysegeschwindigkeiten von Chlormethyl-acyl-sulfiden und der analogen Sauerstoffverbindungen in Wasser-Dioxan-Gemischen werden ermittelt und mit denjenigen von Chlormethyläthern und Chlormethylthioäthern verglichen.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.19596230109
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    Paper (German National Licenses)
    Fulltext
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