ISSN:
1615-6102
Keywords:
Glyoxysomes
;
Leaf peroxisomes
;
Microbody transition
;
Protein A-gold labeling
;
Pumpkin cotyledons
;
Senescence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary After the functional transition of glyoxysomes to leaf peroxisomes during the greening of pumpkin cotyledons, the reverse microbody transition of leaf peroxisomes to glyoxysomes occurs during senescence. Immunocytochemical labeling with protein A-gold was performed to analyze the reverse microbody transition using antibodies against a leaf-peroxisomal enzyme, glycolate oxidase, and against two glyoxysomal enzymes, namely, malate synthase and isocitrate lyase. The intensity of labeling for glycolate oxidase decreased in the microbodies during senescence whereas in the case of malate synthase and isocitrate lyase intensities increased strikingly. Double labeling experiments with protein A-gold particles of different sizes showed that the leaf-peroxisomal enzymes and the glyoxysomal enzymes coexist in the microbodies of senescing pumpkin cotyledons, indicating that leaf peroxisomes are directly transformed to glyoxysomes during senescence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01385011
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