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  • Microprojectile bombardment  (1)
  • Vascular expression  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment (2000)
    Springer
    Plant cell reports 19 (2000), S. 229-234 
    Details
    ISSN: 1432-203X
    Keywords: Key words Musa ; Banana ; Transformation ; Microprojectile bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the β-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. Plants were regenerated, under selection with kanamycin, that were co-transformed with nptII and either the uidA or BBTV genes. Molecular characterisation of transformants demonstrated that the transgenes had been stably integrated into the banana genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050004
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Promoters derived from banana bunchy top virus DNA-1 to −5 direct vascular-associated expression in transgenic banana (Musa spp.) (2000)
    Springer
    Plant cell reports 19 (2000), S. 810-814 
    Details
    ISSN: 1432-203X
    Keywords: Key words Banana bunchy top virus ; Promoter ; Vascular expression ; Green fluorescent protein ; Intron-mediated enhancement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intergenic regions of banana bunchy top virus (BBTV) DNA-1 to -5 were fused to the green fluorescent protein (GFP) and uidA reporter genes and assessed for promoter activity in transgenic banana (Musa spp. cv. Bluggoe). Promoter activity associated with the BBTV-derived promoters was transgene dependent with greatest activity observed using the GFP reporter. The BBTV promoters (BT1 to BT5) directed expression primarily in vascular-associated cells, although levels of activity varied between individual promoters. Promoters BT4 and BT5 directed the highest levels of GFP expression, while activity from BT1, BT2 and BT3 promoters was considerably weaker. Intron-mediated enhancement, using the maize polyubiquitin 1 (ubi1) intron, generated a significant increase in GUS expression directed by the BBTV promoters in transgenic plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002999900185
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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