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  • Microtubules  (2)
  • Polymer and Materials Science  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Coordination of division events in theChlamydomonas cell cycle (1986)
    Springer
    Protoplasma 131 (1986), S. 118-130 
    Details
    ISSN: 1615-6102
    Keywords: DNA inhibition ; Mitosis ; Microtubules ; Phycoplast ; Plant cytokinesis ; Wall deposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary At concentrations that did not affect growth, hydroxyurea and 21-deoxyadenosine inhibited DNA synthesis inChlamydomonas. Evidence that initiation of mitosis is dependent upon completion of DNA replication was provided by the arrest of inhibited cells with undivided nuclei containing undispersed nucleoli. Initiation of cytokinesis is not dependent upon progress of nuclear division since, in arrested cells, cleavage microtubules became deployed in a phycoplast and a cleavage furrow developed fully, until obstructed by the undivided nucleus. Chloroplast constriction and division also continued independently of nuclear division. It is concluded that nuclear division, cytoplasmic cleavage and chloroplast division are in separate sequences of dependent events. This is supported by flexibility of their relative timing in successive divisions, since after the first commitment to divide nuclear division is followed by initiation of cleavage and then chloroplast division, whereas following subsequent commitments these events occur in reverse time order. This flexibility of order indicates changing rates of progress through separate sequences of events. Deposition of wall material was dependent upon the completion of cytokinesis, but this inhibition of wall deposition by incomplete cytokinesis did not extend to other daughters within the same mother cell. These observations are correlated with our earlier data concerning the rate-limiting control points for division and a model for the coordination of division events is presented. The relationships between different plant cell cycles is discussed in view of the findings presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01285034
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and partial characterization of conditional cell division cycle mutants inChlamydomonas (1995)
    Springer
    Protoplasma 186 (1995), S. 149-162 
    Details
    ISSN: 1615-6102
    Keywords: Algae ; Cytoskeleton ; Microtubules ; Microtubule organizing centres ; Mutation ; Temperature-sensitive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated a number of temperature conditional cell division cycle mutants of the unicellular plantChlamydomonas reinhardtii that are defective in single nuclear genes. Cells grow and divide normally at the permissive temperature (21 °C), but arrest in division at the restrictive temperature (33 °C). We have characterized these mutants using DNA probes and immunofluorescence techniques to localize cytoskeletal and microtubule organizing centre proteins. We describe here 3 broad classes of cell cycle mutation which result in cell cycle arrest with: unreplicated DNA (G1 arrest), duplicated DNA (G2 arrest) and multiple nuclei due to defective cytokinesis (cytokinesis arrest). The continuation of nuclear division in mutants blocked in cytokinesis provides support of an earlier hypothesis that stage specific events in theChlamydomonas cell cycle are arranged in separate dependent sequences. The mutants isolated in the present study provide insights into the role of cytoskeletal proteins in the coordination of plant cell division and the means to investigate the molecular mechanisms whereby division by multiple fission is controlled in the unicellular plantChlamydomonas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281325
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  • 3
    Electronic Resource
    Electronic Resource
    Adhesion enhancement of rubbery and ductile polyolefin coatings on glass fibers for epoxy composites and effects on failure mechanism (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 47 (1993), S. 487-498 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Dilute polyolefin solutions, respectively based on EPDM and UHMWPE, were reacted with functionalized silanes to prepare coatings for glass fibers. These were found to provide adequate bonding characteristics for both glass and epoxy resin (bisphenol type). Unidirectional composites containing fiber matrix interlayers, using fibers coated with the aforementioned functionalized polyolefins, were found to exhibit much larger tanδ values over the temperature range of 60 to +60°C and greater ductility in flexural tests, albeit at the expense of a reduction in failure stresses. The UHMWPE coatings produced a multiple step fracture mechanism, which was deemed to provide a more efficient crack stopping mechanism than equivalent EPDM coated fibers. © 1993 John Wiley & Sons, Inc.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1993.070470309
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  • 4
    Electronic Resource
    Electronic Resource
    Interfacial reactions between Al and RuO2, MoOx and WNx diffusion barriers on Si (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 14 (1989), S. 7-12 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The Interfacial reactions between Al and RuO2, MoOx and WNx diffusion barriers on Si (100) wafers have been studied. The diffusion barrier structures were analyzed before and after various heat treatments using Auger electron spectroscopy (AES) and cross-sectional transmission electron microscopy (XTEM). Al was found to reduce the oxides of both Ru and Mo. A 100 Å thick Al2O3 layer developed between the Al and the RuO2 films during annealing at 500°C for 30 minutes. The formation of interfacial Al2O3 efficiently prevents Al penetration but may cause a too high contact resistance for applications in contact structures of microelectronic devices. The WNx barrier was stable after annealing at 600°C for 30 minutes, if the film was exposed to air prior to Al deposition. If, instead, the Al was deposited in situ, the structure failed after annealing at 500°C due to a reaction between Al and W giving WAl12. A thin Al2O3 layer was detected between the Al and the WNx films of the air exposed sample. This layer had the effect of retarding the Al-W reaction and thereby raised the failure temperature of the barrier by at least 100°C.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740140104
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    Paper (German National Licenses)
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