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  • 1
    Electronic Resource
    Electronic Resource
    Sexing of forensic samples using PCR (1991)
    Springer
    International journal of legal medicine 104 (1991), S. 205-207 
    Details
    ISSN: 1437-1596
    Keywords: DNA ; PCR ; Y chromosome ; X chromosome ; Sexing ; DNA ; PCR ; Y-Chromosome ; X-Chromosom ; Geschlechtsbestimmung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Wir haben ein Schnellprotokoll zur Geschlechtsbestimmung forensischer Proben mit Hilfe der „Polymerase Chain Reaction” etabliert. Hierzu werden 3 Primer-Paare verwendet; 2 hiervon sind spezifisch für repetitive Sequenzen auf dem Y-Chromosom, ein Paar ist spezifisch für repetitive Sequenzen auf dem X-Chromosom. Die Methode wird detailliert beschrieben, ebenfalls die durchgeführten Kontrollen. Die Anwendungsmöglichkeiten an Blutflecken und Vaginalabstrichen werden vorgestellt. Erwähnung findet auch die Empfindlichkeit des Tests und die Problematik, welche durch Verunreinigungen der Spur entsteht.
    Notes: Summary A rapid protocol has been established for sexing forensic samples by the Polymerase Chain Reaction method. Three sets of primer were used, two specific for Y chromosome repetitive sequences and one specific for X chromosome repetitive sequences. Detailed procedures of experiments, the controls and the applications to testing bloodstains and a vaginal swab are presented. The sensitivity of the test and problems due to contamination are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01369808
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mitochondrial rps14 is a transcribed and edited pseudogene in Arabidopsis thaliana (1992)
    Springer
    Plant molecular biology 20 (1992), S. 1169-1174 
    Details
    ISSN: 1573-5028
    Keywords: plant mitochondria ; PCR ; cDNA amplification ; editing ; S14 ribosomal protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located upstream of the nad3 locus in O. berteriana. The rps14 locus is present as a single copy in the A. thaliana mt genome and has a translational stop codon located near the initiation codon, as well as a deletion of one nucleotide that disturbs the coding sequence. The cloning and sequencing of nine amplified mt rps14 cDNAs clearly demonstrated that this gene is transcribed and that the mRNA precursors are edited at three positions, all involving C-to-U conversions. No editing events changing the stop codon and restoring the correct coding sequence were witnessed within the 9 individual cDNA clones. Therefore, we conclude that the single rps14 sequence of the mitochondrial genome from A. thealiana is in fact a pseudogene that is transcribed and edited but not translated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028903
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    Paper (German National Licenses)
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