ISSN:
1617-4623
Keywords:
Pathway
;
Gene cloning
;
Karyotyping
;
Mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The four enzymatic steps in the conversion of α-ketoisovaleriate to leucine were examined in the wild type and in 13 leucine auxotrophic strains of Candida maltosa. The genetic lesions in the auxotrophs, involve at least five different loci and are correlated with three enzymatic steps. This was confirmed by gene cloning, protoplast fusion, and enzyme assays. The pathway for leucine biosynthesis in C. maltosa shows general similarity to that of other lower eukaryotes but there are individual differences in the numbers of genes responsible for single enzymatic steps. A disomic state of the chromosomes carrying genes coding for α-isopropylmalate synthase and β-isopropyl-malate dehydrogenase was elucidated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273924
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