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  • 1
    Electronic Resource
    Electronic Resource
    Manipulation of the polyamine content and senescence of apical buds of G2 peas (1985)
    Springer
    Plant growth regulation 3 (1985), S. 401-417 
    Details
    ISSN: 1573-5087
    Keywords: Pea ; Pisum ; apical senescence ; defruiting ; polyamines ; putrescine ; spermidine ; spermine ; polyamine ; synthesis inhibitors ; gibberellin ; 1-aminocyclopropane-1-carboxylic acid ; aminoethoxyvinyl glycine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of various treatments on the apical senescence and polyamine content of apical buds of G2 peas was analysed. Defruiting prevented senescence and increased bud size and polyamine content. Exogenous applications of GA20 enhanced bud size and spermidine concentration. Applied spermidine had a slight effect on spermidine level but did not delay senescence. ACC strongly induced adecrease in bud size and, at 10 mM, apical senescence. This was accompanied by a steady decline in the level of all polyamines though their concentration remained constant until 10 mM ACC, where a drop was noted. Spermidine in the presence of ACC modulated the effect of ACC on the bud size while returning the internal polyamine content to control levels. AVG, an inhibitor of ACC synthesis produced pronounced increases in putrescine though no apparent effect on apical bud growth. Polyamine synthesis inhibitors were without effect on growth or internal polyamine content. The internal polyamine content appeared to correlate with apical bud size and vigor but did not show any consistent relationship to apical bud senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00117596
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Endogenous cytokinins in vegetative shoots of peas (1986)
    Springer
    Plant growth regulation 4 (1986), S. 311-323 
    Details
    ISSN: 1573-5087
    Keywords: Pea ; Pisum ; G2 ; senescence ; cytokinin ; zeatin ; zeatin riboside ; zeatin-0-glucoside ; zeatin riboside-0-glucoside ; dihydrozeatin ; dihydrozeatin riboside ; zeatin-7-glucoside ; zeatin-9-glucoside ; mass spectrometry ; selected ion monitoring ; stable isotope dilution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In G2 peas senescence only takes place in long days. In order to determine the role of cytokinins in this process the endogenous cytokinins from vegetative shoots of G2 peas were characterized using gas chromatography-mass spectroscopy following purification by HPLC. Cytokinins were extracted and purified with and without the addition of 15N labelled internal standards of several cytokinins to estimate cytokin content by isotope dilution in the mass spectra. Samples without internal standards were bioassayed after HPLC. Bioassays showed the presence of zeatin, zeatin riboside and zeatin-0-glucoside. The presence of zeatin was confirmed by its mass spectrum of its permethylated derivative. Tentative identification of zeatin riboside, zeatin-0-glucoside, dihydrozeatin, and dihydrozeatin-0-glucoside was obtained by the coincidence of the major ion for the permethylated natural and 15N labelled internal standards on GC-MS, and the similar coincidence of ions for permethylated zeatin riboside-0-glucoside by direct probe MS. There was no indication of the presence of significant quantities of zeatin-7-glucoside or zeatin-9-glucoside. The amounts in the tissue ranged from 200–1000 ng/kg fresh weight for each cytokinin and about 2–4 μg/kg fresh weight for total cytokinins. There was no apparent difference in the levels in mature but pre-senescent shoots grown in long days and short days indicating that apical senesecence in G2 peas does not appear to be induced by a decline in cytokinin level in the shoots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024931
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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