ISSN:
1573-5028
Keywords:
large spacer of rDNA
;
Pisum sativum
;
repeated DNA
;
S1 nuclease mapping
;
Vicia faba
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants,Vicia faba andPisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which accunt well for the length heterogeneity of the rDNA repeating units. When the entire lengths ofVicia andPisum rDNA large spacers were compared, significant sequence similarity ranging from approximately 50 to 80% was observed, thus a close phylogenetical relationship was revealed. However, organization patterns of these rDNA spacers were clearly different from each other. ThePisum rDNA large spacer contained only one type of repeated sequence of about 175 bp, whereas theVicia contained five kinds of repeated sequence. In addition, the initiation site for transcription was tentatively determined by S1 protection experiment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00019395
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