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  • 1
    Electronic Resource
    Electronic Resource
    Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis (1998)
    Springer
    Plant cell reports 17 (1998), S. 705-710 
    Details
    ISSN: 1432-203X
    Keywords: Key words Organogenesis ; Cotyledon ; Pigeonpea ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 μm N6-benzylaminopurine, 2.3 μm kinetin, and 271 μm adenine sulfate. The shoot buds developed into shoots when they were subcultured on the same medium but with one-tenth concentrations of cytokinins and adenine sulfate. The shoots elongated by subculturing first two to three times on Murashige and Skoog (MS) basal medium supplemented with 2.22 μm N6-benzylaminopurine and 0.54 μm α-naphthaleneacetic acid or on half-strength MS medium containing 2.89 μm gibberellic acid, and then once on the same medium without growth regulators. Elongated shoots were rooted with 80–85% efficiency on MS medium with 4.92 μm indole-3-butyric acid and the plantlets were transferred for hardening. Plants survival in pots was 70–75%. This method may be useful for improving the crop through genetic manipulations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050469
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. (1998)
    Springer
    Plant cell reports 17 (1998), S. 721-725 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPiper colubrinum ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A single-step, high-frequency regeneration pro-tocol has been standardised for Phytophthora-resistant wild pepper, Piper colubrinum (Link) using root, internode, leaf and petiole explants derived from in vitro plantlets. The effect of BA on shoot-bud induction and elongation was assessed by supplementing half-strength MS medium (macronutrients at half the concentration) with different concentrations of BA, i.e. 0.2–10 mg l–1 in induction media and 0, 0.2 and 0.5 mg l–1 in subculture media. The interaction between culture period and BA concentration was studied by culturing the explants for 8, 15 and 30 days before the first subculture. The elongated shoots were rooted directly in soil and hardened in the greenhouse. The developed protocol would be useful in marker-assisted asymmetric hybridisation programmes involving wild-type Piper colubrinum and the cultivated species P. nigrum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050472
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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