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  • 1
    Electronic Resource
    Electronic Resource
    Response of mycorrhizal and nonmycorrhizal Arachis hypogaea to NaCl and acid stress (1996)
    Springer
    Mycorrhiza 6 (1996), S. 145-149 
    Details
    ISSN: 1432-1890
    Keywords: Key words Glomus caledonium ; Peanut ; Acid ; Salt ; Stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The response of peanut to salt (NaCl) and acid (HCl) stress was studied in association with Glomus caledonium, an arbuscular mycorrhizal (AM) fungus. The plants were exposed to salt stress by irrigation on alternate days with 1% or 5% NaCl solutions, or with 0.1 N HCl to induce acid stress. Plant yield almost tripled in mycorrhizal plants compared with nonmycorrhizal control plants. AM inoculation significantly increased plant yield and biomass at 1% NaCl, while at 5% NaCl AM was less effective in alleviating salt stress. Percentage AM colonization was also lowest at 5% NaCl. AM inoculation was found to promote the establishment of peanut plants under acid stress conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720050119
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro plant regeneration from leaf callus inPiper colubrinum Link (1996)
    Springer
    Plant cell reports 16 (1996), S. 215-218 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus-mediated shoot regeneration from leaf explants ofPhytophthora resistant pepper (Piper colubrinum Link.) is described. The effect of basal media composition and growth regulators onin vitro response of explants was evaluated. Shoot buds were induced and elongated on half-strength MS medium containing 2.0 mg l−1 BA and 0.5 mg l−1 NAA , as well as 1.0 mg l−1 BA and 0.5 mg l−1 2,4-D. The shoots were rooted in half-strength MS medium with or without IAA or IBA, and then were transferred to soil with 100% survival.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890870
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.) (1997)
    Springer
    Plant cell reports 16 (1997), S. 647-652 
    Details
    ISSN: 1432-203X
    Keywords: Cotton ; Gossypium hirsutum L. ; Micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction of multiple shoots in cotton (Gossypium hirsutum L. cv. Anjali-LRK 516) has been achieved with cotyledonary nodes devoid of cotyledons and apical meristems. Explants from 35-day-old seedlings yielded the maximum number of shoots (4.7 shoots/explant) using Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine and kinetin (2.5 mg/1 each). Explants from 35-day-old seedlings raised in glass bottles produced a higher number of multiple shoots (8.3 shoots/explant) than those grown in glass tubes and cultured on the same shoot induction medium. Elongation of multiple shoots was obtained on liquid or agar MS basal medium without phytohormones. In vitro shoots were rooted on half-strength agar-solidified MS basal medium or with 0.05 or 0.1 mg/1 naphthaleneacetic acid. Hardening and survival of tissue culture plantlets was 95% under greenhouse conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275508
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Agrobacterium mediated transformation of chickpea (Cicer arietinum L.) embryo axes (2000)
    Springer
    Plant cell reports 19 (2000), S. 235-240 
    Details
    ISSN: 1432-203X
    Keywords: Key words Chickpea ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 mg/l phosphinothricin, depending on the construct used for transformation. Expression of the chimeric GUS gene was confirmed by histochemical localization of GUS activity in regenerated shoots. Resistant shoots were grafted onto 5-day-old dark-grown seedlings, and mature plants could be recovered. T-DNA integration was confirmed by Southern analysis by random selection of putative transformants. The analysis of 4 plantlets of the T1 progeny revealed that none of them was GUS-positive, whereas the presence of the nptII gene could be detected by polymerase chain reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050005
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  • 5
    Electronic Resource
    Electronic Resource
    Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L.) (1993)
    Springer
    Plant cell reports 12 (1993), S. 652-655 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five genotypes of chickpea (Cicer arietinum L.) PG1, PG5, PG12, N59 and C235 were evaluated for induction of somatic embryogenesis. Somatic embryogenesis was induced from immature cotyledons of genotypes PG12 and C235 and immature embryo axes of genotypes PG5, PG12 and C235. Genotypes N59 and PG1 showed no response. The maximum frequency of globular embryo formation occurred in cotyledonary segments on MS medium with 3.0 mg/l 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). Further embryo development was achieved only in somatic embryos derived from cotyledonary segments of genotype PG12. Globular-stage embryos derived from immature embryo axes of PG5, C235, PG12, and cotyledonary segments of C235 dedifferentiated and formed callus. The cotyledonary stage embryos of genotype PG12 germinated on half-strength MS medium supplemented with 1 mg/l zeatin. The regenerated plants were transferred to soil and grown to maturity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232818
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.) (1997)
    Springer
    Plant cell reports 16 (1997), S. 647-652 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cotton ; Gossypium hirsutum L. ; Micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction of multiple shoots in cotton (Gossypium hirsutum L. cv. Anjali-LRK 516) has been achieved with cotyledonary nodes devoid of cotyledons and apical meristems. Explants from 35-day-old seedlings yielded the maximum number of shoots (4.7 shoots/explant) using Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine and kinetin (2.5 mg/l each). Explants from 35-day-old seedlings raised in glass bottles produced a higher number of multiple shoots (8.3 shoots/ explant) than those grown in glass tubes and cultured on the same shoot induction medium. Elongation of multiple shoots was obtained on liquid or agar MS basal medium without phytohormones. In vitro shoots were rooted on half-strength agar-solidified MS basal medium or with 0.05 or 0.1 mg/l naphthaleneacetic acid. Hardening and survival of tissue culture plantlets was 95% under greenhouse conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050295
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  • 7
    Electronic Resource
    Electronic Resource
    Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis (1998)
    Springer
    Plant cell reports 17 (1998), S. 705-710 
    Details
    ISSN: 1432-203X
    Keywords: Key words Organogenesis ; Cotyledon ; Pigeonpea ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 μm N6-benzylaminopurine, 2.3 μm kinetin, and 271 μm adenine sulfate. The shoot buds developed into shoots when they were subcultured on the same medium but with one-tenth concentrations of cytokinins and adenine sulfate. The shoots elongated by subculturing first two to three times on Murashige and Skoog (MS) basal medium supplemented with 2.22 μm N6-benzylaminopurine and 0.54 μm α-naphthaleneacetic acid or on half-strength MS medium containing 2.89 μm gibberellic acid, and then once on the same medium without growth regulators. Elongated shoots were rooted with 80–85% efficiency on MS medium with 4.92 μm indole-3-butyric acid and the plantlets were transferred for hardening. Plants survival in pots was 70–75%. This method may be useful for improving the crop through genetic manipulations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050469
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  • 8
    Electronic Resource
    Electronic Resource
    Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. (1998)
    Springer
    Plant cell reports 17 (1998), S. 721-725 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPiper colubrinum ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A single-step, high-frequency regeneration pro-tocol has been standardised for Phytophthora-resistant wild pepper, Piper colubrinum (Link) using root, internode, leaf and petiole explants derived from in vitro plantlets. The effect of BA on shoot-bud induction and elongation was assessed by supplementing half-strength MS medium (macronutrients at half the concentration) with different concentrations of BA, i.e. 0.2–10 mg l–1 in induction media and 0, 0.2 and 0.5 mg l–1 in subculture media. The interaction between culture period and BA concentration was studied by culturing the explants for 8, 15 and 30 days before the first subculture. The elongated shoots were rooted directly in soil and hardened in the greenhouse. The developed protocol would be useful in marker-assisted asymmetric hybridisation programmes involving wild-type Piper colubrinum and the cultivated species P. nigrum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050472
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  • 9
    Electronic Resource
    Electronic Resource
    Studies on a drought resistant legume: The moth bean, Vigna aconitifolia (Jacq) marechal. I. Protoplast culture and organogenesis (1984)
    Springer
    Plant cell reports 3 (1984), S. 30-32 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract High yields of viable protoplasts were obtained from callus cultures derived from shoot apices of Vigna aconitifolia (JACQ) Marechal. The protoplasts divided and formed cell clusters on modified MS medium. The protoplast-derived callus formed multiple shoot buds on MS and B5 basal media without supplements, on MS medium containing supplements and on B5 medium containing charcoal (0.25%). Shoot formation occurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270225
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  • 10
    Electronic Resource
    Electronic Resource
    Studies on a drought resistant legume: The moth bean, Vigna aconitifolia (Jacq) marechal. II. morphogenetic studies (1984)
    Springer
    Plant cell reports 3 (1984), S. 75-78 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plantlets regenerated from shoot apices, cotyledons and callus cultures in Moth bean, Vigna aconitifolia (JACQ) Marechal, a drought resistant legume and pulse crop, were rooted and transferred to soil. Explants for these studies were derived from seedlings pre-conditioned by germination of seeds on B5BA and WMB (control).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270976
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    Paper (German National Licenses)
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