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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural changes associated with K+-evoked peptide secretion from a neurohemal organ of the crab, Cardisoma carnifex (1985)
    Springer
    Cell & tissue research 242 (1985), S. 67-73 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Sinus gland ; Ultrastructure ; Potassium stimulation ; Crustacea ; Hormone release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron-microscopic comparison of K+-stimulated and unstimulated crab sinus glands reveals significant differences in neurosecretory terminal morphology. Sinus glands exposed to elevated K+ saline for increasing periods of time show increasing numbers of exocytotic release profiles, vacuoles, and multilamellate bodies, and a decrease in the number of microvesicles within 10 μm of release sites. These morphological changes are well correlated with secretion of red-pigment-concentrating hormone, as determined by bioassay of perfusate from the individual preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225564
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in isolated peptidergic nerve terminals induced by digitonin permeabilization and K+ stimulation in the sinus gland of the crab, Cardisoma carnifex (1986)
    Springer
    Cell & tissue research 246 (1986), S. 365-371 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Sinus gland ; Isolated nerve endings ; Potassium stimulation ; Digitonin permeabilization ; Cardisoma carnifex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The preparation of isolated peptidergic nerve terminals from the sinus gland (a neurohemal organ) of the crab (Cardisoma carnifex) is described. In this species the nerve endings can have diameters up to 30 μm. They release neurosecretory material as judged by the decrease in the volumetric density of granules upon depolarization with potassium. Similar results were obtained after permeabilization of the nerve terminals with digitonin, but only in the presence of micromolar concentrations of calcium. This preparation should prove useful in correlating electrical events with other cellular processes involved in stimulus-secretion coupling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215899
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Developmental changes in structure and polypeptide profile of the androgenic gland of the freshwater prawn Macrobrachium rosenbergii (2000)
    Springer
    Aquaculture international 8 (2000), S. 327-334 
    Details
    ISSN: 1573-143X
    Keywords: androgenic gland ; androgenic hormone ; freshwater prawn ; light and transmission electron microscopy ; (Macrobrachium rosenbergii) ; male morphotypes ; sex-differentiation sodium dodecyl sulfate polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Although the mechanism of sex-differentiation in crustaceans has yet to be defined, the androgenic gland (AG) is thought to be the exclusive organ that produces the androgenic hormone (AH) which induces male sexual development. This paper presents results of light and transmission electron microscopy and total protein analysis of androgenic glands from three male morphotypes (orange-claw, orange-blue-claw and blue-claw) of the freshwater prawn, Macrobrachium rosenbergii. Highest protein content (76 µg//AG) was found in the blue-claw morphotype as compared to the orange-blue-claw (45 µg/AG) and the orange-claw morphotype (19 µg/AG). Sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-page) analysis of the cell free extract of the AG from the three morphotypes revealed four polypeptides (∼16, ∼18, ∼23 and ∼26 Kd) which quantitatively increase from the sexually immature orange-claw to the sexually mature blue-claw morphotype. The 16 and 18 Kd polypeptides could be the AHs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009205027360
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    Paper (German National Licenses)
    Fulltext
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