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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 111 (1998), S. 147-150 
    ISSN: 1437-1596
    Keywords: Key wordsPsilocybe mushrooms ; Honey ; Psilocybine ; Psilocine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract In 1996 samples of suspicious honey preparations were confiscated at the Dutch-German border. The labels on the 50 ml jars indicated that the honey contained Stropharia cubensis (better known as Psilocybe cubensis). The jars were filled with honey with a ca. 1 cm layer of fine particles on the top. The particles were collected and subjected to microscopic and chemical analysis. By microscopy mushroom tissue (plectenchym) and spores typical for the genus Psilocybe were identified in all samples. The HPLC analysis with atmospheric pressure mass spectrometry and diode array detection revealed psilocine but psilocybine was not found. The quantitative analysis was very difficult due to the matrix problems. A search showed that the honey with Psilocybe can be purchased in Dutch coffee shops without any limitations although psilocine and psilocybine belong to listed substances according to Dutch law.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1437-1596
    Keywords: Drugs of abuse ; Enzyme multiplied immunoassay (EMIT) ; Fluorescence polarization immunoassay (FPIA) ; Acetone precipitation ; Ultrafiltration ; Betäubungsmittel ; Enzym multiplied immunoassay (EMIT) ; Fluorescenzpolarisation-Immunoassay (FPIA) ; Acetonfallung ; Ultrafiltration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Zum immunologischen Nachweis von Drogen in Blutproben wurde die Brauchbarkeit der ETS (EMIT)- und ADx (FPIA)-Systeme unter Einsatz zweier Probenvorbereitungsmethoden (Ultrafiltration und Acetonfällung) untersucht und die Ergebnisse miteinander verglichen. Cannabinoide (D-8-THC-COOH), Benzodiazepine (Oxazepam) und Benzoylecgonin waren nach Ultrafiltration mit beiden Systemen praktisch nicht nachweisbar. Die Nachweisbarkeit von Morphin war nach Ultrafiltration deutlich schlechter als nach vor-ausgegangener Acetonfällung. Der Vergleich der mit dem ETS- und ADx-System nach Acetonfällung erhaltenen Ergebnisse zeigt, daß der ETS-Assay etwas empfindlichere, der ADx-Assay besser reproduzierbare Meßergebnisse liefert. Beide Assays sind für Blutuntersuchungen geeignet und lassen vergleichbare cut-off-Werte zu. Das ETS-System zeigt in der Praxis eine bessere Handhabung, Geschwindigkeit und Flexibilität sowie im Vergleich zum ADx deutlich niedrigere Reagenzienkosten.
    Notes: Summary The purpose of the paper was to compare the performance of ETS (EMIT) and ADx (FPIA) analyzers for screening blood samples for drugs of abuse after 2 alternative pretreatment procedures (acetone precipitation and ultrafiltration). Cannabinoids, benzodiazepines and benzoylecgonine were not detectable with both assays after ultrafiltration. The detectability of morphine in blood ultrafiltrates was distinctly lower than after acetone precipitation. The comparison of results obtained with ETS and ADx after acetone precipitation showed that ETS assay is slightly more sensitive but AD is slightly more reproducible. Both assays may be used for blood examination with similar cut-off values. The ETS analyzer gave much better analytical performance (speed, flexibility) and lower reagent costs than the ADx analyzer.
    Type of Medium: Electronic Resource
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