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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 177 (1980), S. 33-41 
    ISSN: 1433-8580
    Keywords: IgM ; Purification ; Methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary For the preparation of gram amounts of IgM from human sera sedimention at 100,000g or treatment with ZnSO4 of the redissolved “euglobulin”-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably.
    Type of Medium: Electronic Resource
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