ISSN:
1432-072X
Keywords:
Pyrimidine degradation
;
Pyrimidine uptake
;
Thymine
;
Uracil
;
Dihydrothymine
;
Dihydrouracil
;
β-Ureidoisobutyrate
;
Stickland reaction
;
Clostridium sticklandii
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Resting cells of Clostridium sticklandii took up thymine or uracil, when grown in a medium containing 40 mM serine and 20 mM thymine or uracil. The uptake was much lower, when the cells had been grown in a complex medium. Cell-free extracts from cells grown in the complex medium reduced the two bases to the dihydro compounds and decomposed dihydrothymine to β-ureidoisobutyrate, as indicated by thin-layer chromatography. Uptake and degradation were stimulated by both NADH and NADPH. Further breakdown did not occur, as 14CO2 was not evolved from C-2-labelled thymine or uracil. The rates of pyrimidine uptake and breakdown of C. sticklandii were lower than those reported for C. sporogenes (Hilton et al., 1975).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00407038
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