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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 138 (1973), S. 503-522 
    ISSN: 1432-0878
    Keywords: Median eminence ; Birds ; Monoaminergic innervation ; Radioautography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Dans l'Eminence Médiane du Canard, la Noradrénaline et la Dopamine tritiées sont captées et retenues auniveau de la zone interne et en périphérie des vaisseaux portes hypophysaires. Cette rétention, observée par radioautographie après incubation in-vitro ou injection intraventriculaire des médiateurs, est considérablement diminuée par la réserpine. Une ou deux injections intraventriculaires de 6-Hydroxy-Dopamine laissent subsister quelques fibres capables de capter la Noradrénaline-3H. Après trois injections de 6-Hydroxy-Dopamine, on n'observe plus aucune incorporation du médiateur. Ces données radioautographiques et pharmacologiques attestent que les fibres de la zone interne de l'Eminence Médiane, qui captent la Noradrénaline et ses précurseurs et qui sont détruites par la 6-Hydroxy-Dopamine, sont de nature catécholaminergique. En microscopie électronique, on constate que les fibres marquées contiennent des vésicules de types divers avec fréquemment un coeur dense excentré. Ces fibres présentent des contacts synaptiques avec des dendrites ou péricaryons neuronaux qui ne retiennent pas le traceur et qui reçoivent également d'autres afférences synaptiques non marquées. Ce fait permet d'établir que les neurones infundibulaires présents dans l'Eminence Médiane sont susceptibles d'une régulation mettant en jeu des catécholamines et d'autres types de neurotransmetteurs.
    Notes: Summary When3H-norepinephrine or3H-dopamine are given to the median eminence of the Duck after an injection into the IIIrd ventricle or afterin vitro incubation, an intense and preferential accumulation of the tracer is observed in the internal zone and in the vicinity of the primary portal vessels. The amount of labeled catecholamines in the median eminence is greatly diminished by reserpine. After one or two injections of 6-hydroxy-dopamine into the third ventricle followed by application of3H-norepinephrine, only a few fibers are labeled; after three injections of this drug, nearly no uptake of3H-norepinephrine is seen. This radioautographic and pharmacological approach indicates that the axons of the internal zone of the median eminence, which are able to take up and store labeled norepinephrine and its precursors and which are destroyed by 6-hydroxy-dopamine, are catecholaminergic. The ultrastructural examination of the internal zone shows that the label is confined to axonal varicosities containing various types of vesicles possessing frequently an eccentric core. The labeled axonal varicosities were found in synaptic contact with unlabeled dendrites and also with unlabeled perikarya. The postsynaptic structures which receive labeled presynaptic axons display also synaptic contacts with other unlabeled axons. This fact suggests that neurons of the median eminence are probably modulated by catecholamines and other neurotransmitters either on their dendrites and even on their soma.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Serotonin ; Neuropeptide Y ; Suprachiasmatic nucleus ; Radioautography ; Immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphological substrate of putative serotonin (5-HT)/neuropeptide Y (NPY) interactions in thé suprachiasmatic nucleus (SCN) was investigated by combined radioautography and immunocytochemistry after intraventricular administration of (3H)5-HT in the rat. In the ventral portion of the SCN, the distribution of (3H)5-HT uptake sites overlapped closely the NPY-immunoreactive terminals. Previous investigations have shown that the dense 5-HT and NPY innervations of the SCN originate in different structures, i.e., the midbrain raphe nuclei and the ventral lateral geniculate nucleus, respectively. Accordingly, in the present study, destruction of 5-HT afferents by 5,7-dihydroxytryptamine was not found to induce any modification in NPY staining and, in ultrastructural immuno-radioautographic preparations, two distinct pools of axonal varicosities could be identified. Both 5-HT and NPY terminals established morphologically defined synaptic junctions, sometimes on the same neuronal target. Some cases of direct axo-axonic appositions between the two types of terminals were also encountered. These data constitute additional criteria for characterizing the cytological basis of the multiple transmitter interactions presumably involved in the function of the SCN as a central regulator of circadian biological rhythms.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 238 (1984), S. 621-626 
    ISSN: 1432-0878
    Keywords: Catecholamines ; Pituitary innervation ; Radioautography ; Ultrastructure ; Goldfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The monoaminergic innervation of the goldfish pituitary gland was studied by means of light- and electronmicroscopic radioautography after in vitro administration of 3H-dopamine. The tracer was specifically incorporated and retained by part of the type-B fibers innervating the different lobes of the pituitary. In the rostral pars distalis labeled fibers were most frequently observed in contact with the basement membrane separating the neurohypophysis and the adenohypophysis. In the proximal pars distalis and the pars intermedia, labeled profiles were detected in the neural tissue and in direct contact with the different types of secretory cells. According to the previous data concerning the uptake and retention of tritiated catecholamines in the central nervous system, it is assumed that the labeled fibers are mainly catecholaminergic (principally dopaminergic). This study provides morphological evidence for a neuroendocrine function of catecholamines in the goldfish.
    Type of Medium: Electronic Resource
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