ISSN:
1617-4623
Keywords:
Arabidopsis thaliana
;
β-Glucuronidase
;
Root tip
;
Seed protein gene
;
Vicia faba
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have isolated a novel gene, denoted USP, from Vicia faba var. minor, which corresponds to the most abundant mRNA present in cotyledons during early seed development; however, the corresponding protein does not accumulate in cotyledons. The characterized USP gene with its two introns is 1 of about 15 members of a gene family. A fragment comprising 637 bp of 5′ flanking sequence and the total 5′ untranslated region was shown to be sufficient to drive the mainly seed-specific expression of two reporter genes, coding for neomycin phosphotransferase 11 and β-glucuronidase, in transgenic Arabidopsis thaliana and Nicotiana tabacum plants. We showed that the USP promoter becomes active in transgenic tobacco seeds in both the embryo and the endosperm, whereas its activity in Arabidopsis is detectable only in the embryo. Moreover, we demonstrated a transient activity pattern of the USP promoter in root tips of both transgenic host species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00261688
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