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  • 1
    Digitale Medien
    Digitale Medien
    Physical mapping of translocation breakpoints in rye by means of synaptonemal complex analysis (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 33-41 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Physical mapping Translocation breakpoint ; C-banding Synaptonemal complex ; Rye
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A physical map including 40 translocation breakpoints has been constructed in rye by means of synaptonemal complex (SC) analysis of well-paired pachytene quadrivalents. The chromosome arms involved in such translocations were previously identified either from mitotic C-banding analysis or from the meiotic configurations observed in the progenies of crosses with a rye line having multiple chromosome rearrangements. The synaptonemal complexes formed by some translocation homozygotes were also analyzed, the relative pachytene SC length of their translocated chromosomes being compared to that observed in the corresponding translocation heterozygotes. In the translocations in which the position of the breakpoint could be well defined from mitotic C-banding analysis, a good correspondence between the relative position of the point showing partner exchange in the pachytene quadrivalents and the actual location of the breakpoint was established. It is concluded that the mapping of translocation breakpoints by SC analysis of pachytene quadrivalents provides a more accurate estimate of the position of the breakpoints than that obtained from mitotic C-banding analysis, due to the lack of evenly-distributed interstitial C-bands in most rye chromosomes. The distribution of the breakpoints along the chromosomes in relation to their spontaneous or induced origin is also discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226979
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Genetic mapping of cytological and isozyme markers on chromosomes 1R, 3R, 4R and 6R of rye (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 208-214 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rye ; C-heterochromatin bands ; Isozymes ; Translocation ; Genetic mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cytogenetic maps involving chromosomes 1R, 3R, 4R and 6R have been developed from the analysis of offspring of crosses between multiple heterozygous rye plants. The maps include isozyme loci GpiR1, Mdh-R1 and Pgd2 (located in chromosome 1R), Mdh-R2 (located in chromosome 3R), Pgm-R1 (located in chromosome 4R) and Aco-R1 (located in chromosome 6R). Various telomeric and interstitial C-bands of these four chromosomes, the centromere split of chromosome 3R, and translocation TR01 were used as cytological markers. By means of electron microscope analysis of spread pachytene synaptonemal complexes, the breakpoint of TR01 was physically mapped in chromosome arms 4RS and 6RL. From the linkage data, conclusions were derived concerning the cytological locations of the isozyme loci and the physical extent of the evolutive translocations involving chromosome arm 6RL.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225899
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