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  • 1
    Electronic Resource
    Electronic Resource
    A glycoprotein associated with the acquisition of the self-incompatibility system by maturing stigmas of Brassica oleracea (1979)
    Springer
    Planta 146 (1979), S. 179-183 
    Details
    ISSN: 1432-2048
    Keywords: Brassica ; Cellular recognition ; Glycoproteins ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iso-electric focusing of extracts derived from stigmatic homogenates of Brassica oleracea reveals that the mature stigma possesses large quantities of a glycoprotein not present in earlier stages of development in the bud. Pollen germination experiments carried out in parallel with the biochemical tests suggest that the appearance of this glycoprotein, which has an isoelectric point of pH 5.8, is coincident with the development of the self-incompatibility response. The site of this protein, and the role it may play in pollen-stigma interactions are discussed in terms of current models of the self-incompatibility system in Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388229
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a method for the identification of S alleles in Brassica oleracea based on digestion of PCR-amplified DNA with restriction endonucleases (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 133-138 
    Details
    ISSN: 1432-2145
    Keywords: Brassica oleracea ; Self-incompatibility ; S alleles ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The development of a technique for the identification of S alleles involved in self-incompatibility in Brassica oleracea which is based on the polymerase chain reaction (PCR) amplification of genomic DNA followed by restriction analysis is described. Primers homologous to conserved regions near to the 5′ and 3′ ends of the S coding sequence were used to amplify a number of members of the S multigene family. However, by designing a selective primer and using a higher temperature for annealing in the PCR, we were able to amplify certain members from the multigene family preferentially. These were considered to be the S-locus glycoprotein genes (SLG), since the patterns of restriction bands of the PCR products were shown to correspond to those of the SLG where sequence data were available. DNA samples from plants with certain S alleles were found not to amplify efficiently using the “selective” primers and high annealing temperature. This property, however, could be used as a means of distinguishing plants homozygous for these S alleles, as was demonstrated by an examination of a small F2 population that was segregating for the S5 and S29 alleles. In the investigation of the F2 population, it was found that preferential amplification of one of the alleles of the heterozygotes occurred when the “consensus” primers were used in the PCR. However, by using different primers, homologous to another region of the S sequence, we were able to amplify both alleles of the heterozygotes equally. The genotypes of the plants were determined by restriction analysis of PCR products and agreed with results based on pollen-tube growth tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227658
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  • 3
    Electronic Resource
    Electronic Resource
    A molecular approach to the identification of S-alleles in Brassica oleracea (1994)
    Springer
    Sexual plant reproduction 7 (1994), S. 203-208 
    Details
    ISSN: 1432-2145
    Keywords: Brassica oleracea ; Self-incompatibility ; S alleles ; PCR identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A molecular technique for the identification of S-alleles involved in self-incompatibility has been used to analyse the S-allele reference collection of Brassica oleracea. The reference collection contains nearly 50 different lines each with a different S-allele present in the homozygous state. The technique consists of amplifying by the polymerase chain reaction (PCR) sequences belonging to the S multigene sequence family using a single pair of conserved primers. PCR products are then analysed further by digestion with six restriction enzymes followed by gel electrophoresis of the digestion products. A simple method of estimating the band sizes of the digestion products is described. The S-locus-related sequences can be distinguished from S-locus glycoprotein and S-receptor kinase genes by the restriction patterns. Furthermore, with any one restriction enzyme, several alleles showed the same restriction pattern. Alleles could therefore be grouped together. With two exceptions, each member of the S-allele reference collection showed a unique set of restriction patterns. Investigation of the exceptions using pollen tube growth tests showed that these accessions represented duplications within the collection. This technique therefore provides a simple and useful method for identifying different S-alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232738
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  • 4
    Electronic Resource
    Electronic Resource
    Polymorphism of the kinase domain of the S-locus receptor kinase gene (SRK) in Brassica oleracea L. (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 335-342 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica oleracea ; Self-incompatibility ; SRK ; DNA polymorphism ; PCR-RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  DNA polymorphism of the S-locus receptor kinase gene (SRK) participating in self-incompatibility in Brassica was analyzed by PCR-RFLP and nucleotide sequencing. In the screening of primers for specific amplification of polymorphic DNA fragments of SRK, the best combination was that of a forward primer (PK1) having the nucleotide sequence of the second exon of S6 SRK and a reverse primer (PK4) having the complementary nucleotide sequence of the fifth exon of S6 SRK. PCR using this primer pair amplified DNA fragments of 0.9–1.0 kb from 36 S haplotypes out of 42 tested. These DNA fragments showed high polymorphism in polyacrylamide-gel electrophoresis after digestion with restriction endonuclease(s): 25 types were found in a double digestion with MboI and AfaI. Nucleotide sequencing of the DNA fragments amplified from five S haplotypes showed that the third, fourth, and fifth exons of SRK are highly conserved, and that there are high variations of the second and third introns of SRK, which produced polymorphism of the band pattern in PCR-RFLPs. Another forward primer (PK5) having the nucleotide sequence of the second exon, which is derived from S2 SRK, amplified DNA fragments of almost the same region of SRK from 27 S haplotypes in combination with PK4. Although SRK alleles of the class-II S haplotypes were not amplified, all of the class-I S-haplotypes were amplified with a primer mixture of PK1, PK4 and PK5. The DNA fragments of both SRK alleles in S heterozygotes, or a 1 : 1 mixture of the genomic DNA of different S homozygotes, were amplified without exception, suggesting the usefulness of these primers for the identification of S heterozygotes. The DNA fragment sizes obtained by digestion with restriction endonucleases served as markers for the identification of S haplotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050568
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of S tester lines in Brassica oleracea: polymorphism of restriction fragment length of SLG homologues and isoelectric points of S-locus glycoproteins (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1329-1334 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica oleracea ; S haplotype ; Self-incompatibility ; RFLP ; IEF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Forty three S tester lines of Brassica oleracea were characterized using DNA and protein gel-blotting analyses. DNA gel-blot analysis of HindIII-digested genomic DNA with class-I and class-II SLG probes revealed that 40 lines could be classified as class-I S haplotypes while three lines could be classified as class-II S haplotypes. The band patterns in the S tester lines were highly polymorphic. Although the S tester lines typically showed two bands corresponding to SLG and SRK in the analysis with the class-I SLG probe, only one band was observed in the S 24 homozygote. This band was identified as SRK, suggesting that this haplotype has no class-I SLG band. In the analysis using the class-II SLG probe, one plant yielded a different band pattern from the known class-II haplotypes, S 2 , S 5 and S 15 . Unexpectedly, this plant was reciprocally cross-incompatible with the S 2 haplotype. Therefore, it was designated as S 2-b . We found an S 13 haplotype having a restriction fragment length polymorphism different from that of the S 13 homozygotes of the S tester line. These findings indicate that S homozygous lines with the same S specificity do not necessarily show the same band pattern in the DNA gel-blot analysis. Soluble stigma proteins of 32 S homozygotes were separated by isoelectric focusing and detected using anti-S 22 SLG antiserum. S haplotype-specific bands were detected in 27 S homozygotes but not in five S homozygotes, including the S 24 homozygote. This is consistent with the observation that the S 24 haplotype had no SLG band.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051199
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of hexane and humidity on self-incompatibility in Brassica oleracea (1978)
    Springer
    Theoretical and applied genetics 52 (1978), S. 113-117 
    Details
    ISSN: 1432-2242
    Keywords: Self-incompatibility ; Brassica ; Humidity ; Hexane ; Flower Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of hexane, high humidity, flower age and temperature in overcoming the self-incompatibility of Brassica oleracea were studied using three plants, each of which was homozygous for a different dominant S-allele. Hexane had a significant effect in all cases, but the size of the effect varied considerably. In one plant there was a marked interaction between the effect of hexane, humidity and flower age, but temperature had relatively little effect. In another plant high humidity alone gave a very much greater response than hexane alone. This plant gave as many self-seeds from the high humidity treatment as from bud selfing, indicating that the incompatibility reaction was almost completely overcome by the high humidity. The results are discussed in the light of current views of the mechanism of incompatibility in Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264743
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  • 7
    Electronic Resource
    Electronic Resource
    Time of cross- and self-pollination affects the amount of self-seed set by partially self-incompatible plants of Brassica oleracea (1978)
    Springer
    Theoretical and applied genetics 52 (1978), S. 233-237 
    Details
    ISSN: 1432-2242
    Keywords: Self-incompatibility ; Time of pollination ; Pollen tube Competition ; Seedling markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relative ability of cross- and self-pollen to achieve fertilisation in Brassica was studied by making double pollinations using cross-pollen carrying a dominant seedling marker gene. With simultaneous self- and crosspollination 12–40% self-seed was set, but when cross-pollen was applied to the stigma four hours before self-pollen, only 2–4% self-seed was obtained. In two plants to which cross-pollen was applied at various time intervals after self-pollen there was a tendency for the percentage of self-seed to increase as the time interval increased. In a third plant this trend was not apparent, probably because of a greater degree of self-incompatibility. The competitive advantage of the first pollen to arrive on the stigma is discussed in relation to the strength of the self-incompatibility and the sib problem in F1 hybrid brassicas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273895
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  • 8
    Electronic Resource
    Electronic Resource
    Pollen stigma interactions in Brassica oleracea (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 241-246 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Pollen germination ; Pollen surface ; Self-incompatibility ; Stigma surface
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recent studies on the mechanism of self-incompatibility in Brassica indicate the location, nature and mode of action of the molecules involved. Characteristics of the pollen surface and the stigma surface are described in detail, together with new information pertaining to the recognition molecules located therein. A sequence of events is outlined leading from pollination, through adhesion, hydration, germination, and tube growth to acceptance and ultimate compatibility. The characteristics of rejection of incompatible grains are described for each stage of the pollen-stigma interaction. It is proposed that recognition of proteins from the coating of self-pollen by the molecules in the pellicle results in the formation of a biologically-active complex which inhibits water supply to the incompatible grain, and that all other manifestations of incompatibility are a consequence of this initial response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265173
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