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  • 1
    Electronic Resource
    Electronic Resource
    The efficacy of soybean inoculation on acid soil in tropical Africa (1980)
    Springer
    Plant and soil 54 (1980), S. 95-106 
    Details
    ISSN: 1573-5036
    Keywords: Acid soil ; Antibiotic resistance ; Competition ; Rhizobium ; Soybean ; Symbiotic nitrogen fixation ; Tropical Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficacy of inoculating soybean cvs. TGm294-4 and TGm80 in an acid soil (pH 4.6, water) was studied in a field trial in Nigeria.Rhizobium japonicum strains CB1809str r and 46spc r which had been selected for symbiotic competence in the acid soil were used as inocula. Other treatments included no inoculation, combined nitrogen (150 kg N ha−1) and lime (1 t ha−1). Plants grown in acid soil without inoculation and lime had very few nodules, were nitrogen deficient and grain yields were poor (0.3–0.4 t ha−1). Yield responses to combined nitrogen were generally small due to fertilizer loss through leaching. Inoculation greatly increased nodulation, shoot dry matter and shoot N%; grain yields were between 1.6 and 2.0 t ha−1. Lime improved all harvest measurements in the uninoculated and nitrogen treatments but not in the inoculated treatments. In a duplicate trial without lime on near-neutral soil, similar inoculation responses were obtained at 6 weeks. However, at final harvest there were no significant differences in grain yield between treatments due to late, effective nodulation by unidentified strains in the uninoculated treatments. Assays for strain identity confirmed the successful establishment of the inocula in both soils, with 100% of nodules from inoculated plants containing an introduced strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182002
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  • 2
    Electronic Resource
    Electronic Resource
    Mineral N effects on cowpea and soybean crops in a Nigerian soil (1982)
    Springer
    Plant and soil 68 (1982), S. 171-181 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Cowpea ; Fertilizer-N ; Glycine max ; Nitrogen-15 ; Nodule ; Soybean ; Vigna unguiculata ; Yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a Nigerian soil depleted in available N, fertilizer-N enriched in15N was applied at 25 and 100 kg N/ha to crops of four cowpea and two soybean cultivars. Soil-N availability was estimated with three non-fixing crops, non-nodulating soybean, maize and celosia. With sequential harvests we examined the development of the fixing crops, as well as their nodulation profiles and acetylene reduction activities, and the patterns obtained were correlated with uptake of mineral-N. At low levels of mineral-N, excellent nodulation (up to 580 mg dry weight/plant) and very high acetylene reduction activities (up to 208 μmoles/plant/h) were recorded. Although fertilizer-N utilizations were low, 12% and 28% at 25 and 100 kg N/ha respectively, the lower application had a transient adverse effect on nodule development and the higher application had a long-term adverse effect on nodule formation, nodule development and acetylene reduction activity. Cowpea nodule mass reached maximum levels at early pod-fill except at 100 kg N/ha at which it continued to increase into late podfill. In contrast, soybean nodulation did not peak at any level of N but increased into late pod-fill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02373703
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  • 3
    Electronic Resource
    Electronic Resource
    Mineral N effects on cowpea and soybean crops in a Nigerian soil (1982)
    Springer
    Plant and soil 68 (1982), S. 183-192 
    Details
    ISSN: 1573-5036
    Keywords: ‘AN’-value ; Cowpea ; Difference method ; Fertilizer-N ; Glycine max ; Nitrogen-15 ; N-balance ; N2-fixation ; Soybean ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amounts of N fixed in the field in Nigeria by four cowpea and two soybean cultivars were determined at different levels of available N by the difference and the ‘AN’-value methods using non-nodulating soybean, maize and celosia as non-fixing controls. No significant differences were found between the two methods or with different controls. Grain removal and return of residues to the soil were measured and net soil-N changes calculated. N-benefits accrued from two of the cowpea cultivars when grown at low mineral-N levels, but with higher mineral-N availability, no cultivar contributed N because of inhibition of nodulation and lowered N2-fixation. Although the soybeans fixed more N than the cowpeas they caused greater net N-depletions because they sequestered a greater fraction of N in the grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02373704
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  • 4
    Electronic Resource
    Electronic Resource
    Nodulation and growth ofLeucaena leucocephala (Lam.) de Wit as affected by inoculation and N fertilizer (1988)
    Springer
    Plant and soil 112 (1988), S. 129-135 
    Details
    ISSN: 1573-5036
    Keywords: alfisol ; inoculation ; Leucaena leucocephala ; nitrogen fertilizer ; nitrogen fixation ; nodulation ; Rhizobium ; shoot growth ; total nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Leonard jar, pot and field experiments examined the effects of inoculation and the influence of nitrogen fertilizer on nodulation, nitrogen fixation and growth ofLeucaena leucocephala (Lam.) de Wit at IITA, Ibadan, Nigeria. Leucaena responded to both inoculation and/or nitrogen application. Shoot growth and total N and P of inoculated plants were comparable to those of the highest N treatment, and the values were about 55% greater than those of uninoculated ones. Field data indicated that toal N yields of inoculated leucaena were increased by 50% with 40 or 80 kg ha−1 of N fertilizer. However, N fertilizer depressed N fixation by 56% as was expected from nodule mass data. N-fixation was delayed for about 8 weeks in the plots without N. Application of small amounts of N starter (20 ppm) proved to be beneficial to satisfy the plant need during the early stage of leucaena growth. The rhizobial strains IRc 1045 and IRc 1050 were effective, competitive and survived well in the field one year after their establishment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181762
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  • 5
    Electronic Resource
    Electronic Resource
    Response of Leucaena/Rhizobium symbiosis to mineral nutrients in Southwestern Nigeria (1988)
    Springer
    Plant and soil 112 (1988), S. 121-127 
    Details
    ISSN: 1573-5036
    Keywords: alfisol ; allantoin ; inoculation ; Leucaena leucocephala ; mineral nutrition ; nodulation ; nitrogenase activity ; shoot growth ; total nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pot and field experiments carried out at the International Institute of Tropical Agriculture (IITA) and at Fashola, Southwestern Nigeria, examined the effect of inoculation and N, P and micronutrients on nodulation and growth ofLeucaena leucocephala (Lam.) de Wit. In pot studies all parameters measured, except the percentage of nitrogen in shoots, were improved by inoculation, nitrogen and phosphorus. Micronutrients increased only nitrogen and allantoin contents. Interactions between inoculation and P, N and micronutrients on nodulation and growth of leucaena were observed. The effect of inoculation and fertilization with phosphorus or micronutrients was further investigated in field experiments. Establishment of uninoculated and unfertilized leucaena was poor at both locations due to low soil fertility and the presence of only a few native leucaena rhizobia. At one site, only inoculated plants were nodulated, while at the other, all plants produced nodules. Shoot dry weight, total nitrogen and phosphorus of inoculated plants were statistically equal to nitrogen-fertilized plants. Uninoculated plants were stunted. Generally, micronutrients did not influence nodulation, total nitrogen or growth of leucaena. They had only a positive effect on nitrogenase activity. Phosphorus increased total nitrogen and phosphorus uptake and plant growth. A 75% increase in shoot dry weight was obtained when 80 kg P ha−1 was applied to inoculated leucaena with Rhizobium strain IRc 1045. Inoculated plants contained more allantoins than uninoculated ones but no significant correlation was found between these compounds and other parameters of N fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181761
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  • 6
    Electronic Resource
    Electronic Resource
    Nitrogen contribution of Leucaena/Rhizobium symbiosis to soil and a subsequent maize crop (1988)
    Springer
    Plant and soil 112 (1988), S. 137-141 
    Details
    ISSN: 1573-5036
    Keywords: alfisol ; egbeda series ; Leucaena leucocephala ; inoculation ; maize ; nitrogen contribution ; nodules ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The nitrogen contribution from the shoot and root system of symbiotically grown leucaena was evaluated in a field experiment on an Alfisol at IITA in Southern Nigeria. Maize in plots that received prunings from inoculated leucaena contained more N and grain yield was increased by 1.9 t.ha.−1. Large quantities of nitrogen were harvested with leucaena prunings (300 kg N ha−1 in six months) but the efficiency of utilization of this nitrogen by maize was low compared to inorganic N fertilizer (ammonium sulphate) at 80 kg N ha−1. Maize yield data indicated that nitrogen in leucaena prunigs was 34 and 45% as efficient as 80 kg N ha−1 of (NH4)2SO4 for uninoculated and inoculated plants with Rhizobium IRc 1045, respectively. In plots where the prunings were removed, the leaf litter and decaying roots and nodules contributed N equivalent of 32 kg ha−1. Twenty-five kg ha−1 was the inorganic N equivalent from nitrogen fixed symbiotically by leucaena when inoculated with Rhizobium strain IRc 1045. Application of prunings from inoculated leucaena resulted in higher soil ogranic C, total N, pH and available NO3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181763
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