ISSN:
1432-136X
Keywords:
Protein metabolism
;
Ageing rat
;
2-Mercaptoethanol
;
Stable isotope
;
15N-Glycine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Female RLEF1/Lati rats were chronically treated with 2-mercaptoethanol in a dose of 13 μg·100 g bw-1·day-1 dissolved in drinking water. During a 48-h experiment 15N-labelled glycine was given orally in a dose of 5 mg 15N·kg bw-1 and urine samples were collected and analysed by an emission spectrometric isotope method. Protein synthesis and nitrogen excretion rate constants were calculated according to the three-pool model, and 3-methylhistidine excretion rates were also determined. 2-Mercaptoethanol appears to influence protein metabolism; however, the slower rates of protein synthesis proved to be apparent in almost all groups of treated rats. Protein synthesis and nitrogen excretion rate constants have exceptionally high values in 2-year-old rats, possibly explained by the occurrence of hypercompensation mechanisms in old age. These were reflected by the excretion rates of 3-methylhistidine which were reduced as a result of sulphhydryl group interactions in age-dependent cellular metabolic changes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00264816
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