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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 47 (1982), S. 457-460 
    ISSN: 1432-1106
    Keywords: Vision ; Visual cortex ; Complex cells ; Relative motion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Responses of superficial-layer, texturesensitive complex cells in cat striate cortex to relative motion between an oriented bar stimulus and its textured background were recorded. Some cells responded best to motion in one particular direction across the receptive field of the cell, irrespective of whether the bar and background moved simultaneously in the same (in-phase) or opposite (antiphase) directions. Others showed a clear preference for either in-phase or antiphase relative motion, irrespective of direction of motion across the receptive field.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 72 (1988), S. 1-20 
    ISSN: 1432-1106
    Keywords: Vision ; Visual cortex ; Adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Motion after-effects were elicited from striate cortical cells in lightly-anaesthetized cats, by adapting with square-wave gratings or randomly textured fields drifting steadily and continuously in preferred or null directions. The time-course and recovery of responsiveness following adaptation were assessed with moving bars, gratings or textured fields. Results were compared with controls in which the adapting stimulus was replaced by a uniform field of identical mean luminance, and also assessed in relation to the strength and time course of adaptation. Within 30–60 s adaptation, firing declined to a steady-state. Induced after-effects were direction-specific, and manifest as a transitory depression in response to the direction of prior adaptation, recovering to control levels in 30–60 s. Maximal after effects were induced by gratings of optimal drift velocity and spatial frequency. With rare exceptions after-effects were restricted to driven activity; no consistent effects on resting discharge were observed. The onset of adaptation, and the recovery period, were more rapid in simple cells, although after effects of comparable strength were elicited from simple and from standard complex cells. Special complex cells, including many of the more profoundly texture-sensitive neurones in the cortex, were more resistant to adaptation. The results support the conclusion that psychophysically measured adaptation and induced motion after-effect phenomena reflect the known properties of cortical neurones.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 86 (1991), S. 324-332 
    ISSN: 1432-1106
    Keywords: Vision ; Optokinetic nystagmus ; Eye movements ; Plaids ; Brainstem ; Motion perception ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have recorded the direction of optokinetic nystagmus (OKN) elicited by moving plaid patterns in order to dissociate the pathways that mediate horizontal OKN. The plaids used comprised two drifting sinusoidal gratings arranged such that their individual directions of drift were very different from the direction of coherent motion of the overall pattern. The direction of OKN with binocular viewing was close to the mean of the component directions, suggesting a dominant influence of cortical visual neurons that respond to oriented one-dimensional components of the image. But the direction of OKN was consistently shifted slightly towards the direction of motion of the overall pattern, suggesting a secondary influence responsive to pattern direction. OKN recordings obtained during monocular viewing suggest that this secondary influence reflects the direct retinal pathway to the brainstem structures mediating OKN.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 60 (1985), S. 411-416 
    ISSN: 1432-1106
    Keywords: Vision ; Motion after-effects ; Cat ; Visual cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Responses of striate cortical neurones to bars of optimal orientation and width, moving with fixed velocity, were recorded in the lightly anaesthetized cat. Effects of periods of pre-adaptation with square-wave gratings of variable spatial frequency and velocity, drifting continuously in each cell's preferred or null directions, were investigated. Variations of cells' directional bias and responsiveness to oriented bars were assessed in relation to the degree and time-course of pre-adaptation to drifting gratings, compared with the preceding level of firing when exposed to uniform backgrounds of the same average luminance. All cells showed some susceptibility to pre-adapting moving gratings: subsequent responses to a bar were initially depressed in the direction of pre-adaptation and, in direction-biased or bidirectional cells, were enhanced in the opposite direction, compared with bar responses following exposure merely to a uniform background. These effects were strongest and most consistent amongst standard complex cells and weakest amongst special complex cells: maximal effects were obtained with adapting gratings of optimal velocity and spatial frequency.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5087
    Keywords: Arabidopsis ; EBP ; ethylene ; phosphorylation ; receptors ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Previous work in our laboratory has demonstrated the existence of high affinity binding sites for the plant growth regulator ethylene. The ethylene binding protein (EBP), from Phaseolus cotyledons, shows many of the characteristics of a functional receptor for ethylene, has been purified on SDS-PAGE and polyclonal antibodies raised in rabbits. Current work involves the investigation of the ethylene transduction signal in a number of ethylene-responsive tissues. In peas, it has been shown that ethylene promotes the phosphorylation of specific proteins of similar molecular weight to the EBP from Phaseolus. Such ethylene-induced phosphorylation can be inhibited by the ethylene antagonist, 2,5-NBD. The antibodies raised to the EBP from Phaseolus have been shown to immunoprecipitate 32P-labelled proteins from membrane protein preparations obtained from pea tissue. Studies on ethylene binding in pea have also shown that the binding of ethylene may be regulated by phosphorylation. Thus, under conditions which promote phosphorylation, binding is inhibited, whereas the reverse is true under conditions which enhance dephosphorylation. Further work is described which examines the effect of protein kinase, protein phosphatase and calcium channel inhibitors on ethylene-induced phosphorylation in peas, together with wild-type (WT) and ethylene insensitive (eti) mutants of Arabidopsis thaliana. The effects of these treatments can be monitored in vivo using the ethylene-induced triple response as a screen. Furthermore, the protein profiles of such treated seedlings can then be compared by labelling protein extracts with 32P and subjecting the samples to SDS-PAGE followed by autoradiography.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5087
    Keywords: Vicia faba ; ethylene ; ethylene oxide ; waterlogging ; metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The ability of cultivars of Vicia faba L. to metabolize ethylene and the resistance of such cultivars to waterlogging was assessed. Those cultivars showing the highest resistance to waterlogging also had the ability to rapidly metabolize ethylene. It is suggested that a causal relationship may exist between the two processes.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant growth regulation 18 (1996), S. 71-77 
    ISSN: 1573-5087
    Keywords: Arabidopsis ; ethylene ; ethylene binding protein ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A review of work carried out on ethylene binding in higher plants is presented. The use of radio-labelled displacement assays has identified specific 14C-ethylene binding in all tissues so far studied. virtually all higher plants studied contain at least two classes of ethylene binding site, one of which fully associates and dissociates in about 2 h and a class of sites that takes up to 20 h to become fully saturated. Although the types of site differ in their rate constants of association they have similar and high affinities for ethylene. A series of Arabidopsis thaliana mutants shown to vary in sensitivity to ethylene have been analysed for 14C-ethylene binding. One mutant, eti 5, which was shown to be unaffected by ethylene concentrations of up to 10,000 μL L−1 was also shown to exhibit reduced binding. In vivo and in vitro studies on pea have shown that ethylene binding can be detected in this tissue. In vitro studies have shown that both membrane and cytosolic fractions contain measurable amounts of ethylene binding. Interestingly, cytosolic ethylene binding consisted only of the fast associating/dissociating type. Developing cotyledons of Phaseolus vulgaris contain a higher concentration of ethylene binding sites that other tissues and only contain the slow dissociating component. These facets have allowed the purification of ethylene binding protein(s) (EBP) from this tissue. The proteins which bind ethylene can be resolved into two bands of 26 and 28 kDa on semi-denaturing PAGE and the proteins appear to be single entities on a 2-D gels. Data will be presented which indicate a possible role for heterotrimetric G-proteins in the early stages of the ethylene signal transduction pathway.
    Type of Medium: Electronic Resource
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