ISSN:
1432-1912
Keywords:
Key words 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
;
O6-methylguanine
;
K-ras mutation
;
Vitamin E
;
Lung tumorigenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In order to estimate the effect of vitamin E on DNA injury and K-ras point mutation at an early stage of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK)-induced lung tumorigenesis in mice, the present study was carried out. Presupplement with vitamin E about 15 times more than control for a week significantly inhibited NNK-induced O6-methylguanine formation in the lungs of mice at 4 and 168 h after the injection. At 30 days after the NNK injection, the activation of K-ras oncogene with a 12th codon GC→AT transition was detected in 56% of lung samples tested by mutant-allele-specific amplification. Vitamin E supplement reduced the frequency of the mutation to 30%. These results suggest that vitamin E suppresses NNK-induced DNA injury and subsequent fixation of the injury during the initiation and post-initiation phases of the lung tumorigenesis in mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00005253
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